...ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original creators...interrogation of multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve...DNA library preparation. However, when a method relies on accurate ligation of nicks lacking gaps or mismatched...

...2011. 12(2): p. 79-89. PubMed PMID: 21252997. 37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in...2016. 17(7): p. 413-25. PubMed PMID: 26979497. 38. Thorel, F., et al., Conversion of adult pancreatic alpha-cells...Models for Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem...

...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

...inhibited by the unfolding of LOV, allowing the release of Cas9. A noteworthy application of the CASANOVA...in U2OS cells. Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...

...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...page is to make it easier for you to find content relevant to your research and career goals, or to answer...

...Cervical Cancer is the leading cause of cancer related morbidity and death among females in Ghana and ...for cervical cancer development and also better correlate the presence of certain HPV subtypes with risk...

... is in a top-tier publication. Accessibility is surely also behind the increase in citations for older...scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org calls to expand...

... may need to sonicate your lysates in order to release the proteins from their binding. If you’re seeing...technical replicate (ie, leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget...migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of vastly...

...protein domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and ...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells."...

...may help you identify relevant protocols and experimental data Find other related plasmids in the "Associated...

...beneficial effects on the brain,” Ruas said in a press release. “We actually found the opposite: well-trained ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...

... I actually started at a later date and worked entirely virtually. Stepping into this new virtual world... departments of the company. I asked questions relating to their roles at Addgene. I had a great time ...

...up hobbies (for her its gardening) that help you relax and enjoy life. “I love academics because it offers...other's accomplishments? Any tips on making time to relax and/or enjoy time away from the bench? We would ...

...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

...thinking about how I could fill this time. I was relieved to find out that the two hours were split up between...gives a presentation on what they do and how it relates to other teams at the company. This provides new...

...Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists ...resources on the Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19...

...inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors....2019.02.037 Kagiampaki, Z., Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss,...

...  It was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference... all of the research centers in Boston.  I was relieved because it finally feels to me like the postdocs...

... for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light to produce...when in doubt, read the methods sections of the relevant papers, and then read them again. Most of the ...