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We narrowed to 556 results for: SPR

Showing: 381 - 400 of 556 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...the best promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments...NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...later. The PNS presents a different problem: it’s spread throughout the body and is often difficult to surgically...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library...

  4. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    ...culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...covered systemic capsids, which allow AAVs to broadly spread throughout an organism, including difficult-to-...., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

  16. Antibodies 101: Validation

    Type
    Blog Post
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

  18. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...without telling you anything about outliers or data spread within those data sets. Measures of dispersion...allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve) or skewed...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience community like wildfire...identification of opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5...
Showing: 381 - 400 of 556 results