We narrowed to 728 results for: STO;
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TypeBlog Post...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...
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3 Challenges in Plant Synthetic Biology
TypeBlog Post...involved in central anthocyanin metabolism are well understood. Additional work has also identified and characterized...how you were able to overcome them. Share your stories in the comments below! And if you have developed... -
Antibodies 101: Fab Fragments
TypeBlog Post...technique for monoclonal antibodies. J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that...the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...interactions in cells because the protein binders can be customized for protein visualization, relocation, degradation... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...neurons whose function and connectivity are poorly understood,” says Qiu. Qiu developed a dual virus system... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
The time and cost required to make a plasmid
TypeBlog Post...closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time and cost ...any of the numbers in this post is a mathematical story well beyond my ability to tell. But even from these... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending this...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...Until recently, no such tool existed for rapid, customizable protein degradation. Enter TRIM-Away - an antibody-based... super-resolution microscopy.” Endosomes are historically difficult to study in living cells using conventional... -
Developing Lab Management Software for Biology
TypeBlog Post...little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...induces recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks...Cre is absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... -
A Look at Addgene's QC Process
TypeBlog Post...Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains...