We narrowed to 452 results for: SUP

1. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...stable expression of the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis...

2. An Introduction to Adenovirus

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   Blog Post

   ...Persistent infection by adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136...

3. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...with colleagues at other institutions to form a support network called the MDH CUREs Community (MCC) that...

4. Plasmids 101: Colony PCR

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   Blog Post

   ...reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template; and 3) run your...

5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

   ...oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences. Thus...

6. QC Sequencing Technologies at Addgene

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   Blog Post

   ...fraction of one of the four bases (A, C, T, or G) is supplied as a dideoxyribonucleoside triphosphate (ddNTP...

7. Plasmids 101: Shuttle Vectors

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   Blog Post

   ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

8. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ... resources As Addgene built out our scientific support services, we realized that another availability...

9. Hot Plasmids Spring 2024

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   Blog Post

   ...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

10. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ... imaging technologies such as array tomography, super-resolution STORM imaging, and electron microscopy...

11. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...out as a student, people tend to respond in a supportive way and are willing to help because you are not...

12. Hot Plasmids - August 2020

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    ...of bacteria where it can be secreted into the supernatant. Four plasmids containing different nanobodies...

13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

14. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...

15. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected...

16. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...or a magnet (magnetic beads), providing a solid support while unbound proteins are washed away.  While ...

17. Viral Vectors 101: Pseudotyping

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    ...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor...

18. Viral Production at Addgene

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    ...Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

19. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

20. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa in primary rat...