We narrowed to 447 results for: SUP

1. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

   ...oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences. Thus...

2. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by...

3. Hot Plasmids - August 2020

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   ...of bacteria where it can be secreted into the supernatant. Four plasmids containing different nanobodies...

4. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

5. Neuronal labeling with Spaghetti Monster

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   ... imaging technologies such as array tomography, super-resolution STORM imaging, and electron microscopy...

6. Transferable Skills Guide: External Collaborations

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   ...out as a student, people tend to respond in a supportive way and are willing to help because you are not...

7. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

8. New Tools Enable CRISPRa for Neuroscience Applications

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   ...mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa in primary rat...

9. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   ...number of systems that can be studied by providing support "for development of tools, approaches, and infrastructure...

10. Lab to Office Culture Shock

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    ...anymore, but you will very likely be on a team that supports you when you need it. The opportunity to be ...

11. New CRISPR Tools: Cas7-11 and PASTE

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    ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected...

12. Antibodies 101: Immunoprecipitation

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    ...or a magnet (magnetic beads), providing a solid support while unbound proteins are washed away.  While ...

13. Viral Vectors 101: Pseudotyping

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    ...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor...

14. Plasmids 101: Shuttle Vectors

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    ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

15. Twenty Years of Sharing: Expanding Accessibility

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    ... resources As Addgene built out our scientific support services, we realized that another availability...

16. Hot Plasmids Spring 2024

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    ...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

18. Viral Production at Addgene

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    ...Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

19. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...

20. Using Video to Share Your Science: We Share Science

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    Blog Post

    ...years. Which leads to the question of how we can support researchers as they try to keep up with the growing...