Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 561 results for: Spr;

Showing: 381 - 400 of 561 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski... Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    ...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

  4. Five Popular Model Organisms

    Type
    Blog Post
    ... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., B.E., Gradinaru V. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nature...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...without telling you anything about outliers or data spread within those data sets. Measures of dispersion...allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve) or skewed...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

  13. Plasmid Collections

    Type
    Collection
    ...plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors... Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent...pooled libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids...view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience community like wildfire...identification of opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type...

  15. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...subretinal administration and enhanced transduction and spread following injection in the brain. The AAV2(4pMut...displays highly efficient transduction and lateral spread in the retina following subretinal injection. It...serotype has increased transduction and lateral spread in the retina following subretinal injection as...AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep 15;8:184...

  16. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing...Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources Site-specific recombinase...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute to... theses chimaeras to produce all-iPSC derived offspring demonstrates the ability of the iPSCs to form ...

  18. Deep Dive: qPCR

    Type
    Blog Post
    ...analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These useful tools... vol. 1160, DOI 10.1007/978-1-4939-0733-5_3, © Springer Science+Business Media New York 2014Bustin S; ...

  19. Distribution to Industry

    Type
    Collection
    ...Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation...Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1...-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9...
Showing: 381 - 400 of 561 results