...country.   Figure 1: Number of countries Addgene distributes to per year, 2005-2023. We identified and offered... we contracted with so we can always offer the fastest shipping. However, there were issues that persisted... country are represented in blue; darker blue indicates more views from that country.   While written ... country are represented in blue; darker blue indicates more views from that country. Stats and figures...resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit thread crowdsourcing...

...chemiluminescent substrates are available. Labs with limited equipment often opt for the chromogenic substrates since...antibody buffer components can interfere with conjugates. Make sure to do your research before conjugating...sandwich ELISA. In this method, the target antigen competes with a reference antigen for binding to a primary...is non-specific binding or false positives.  If testing a recombinant protein, especially one with tags...

...factor) promotes editing (Yan, J et al., 2024). They developed a new prime editor that incorporates La’s ...AAV Vector System Neurodegeneration Collection updates   Multiplexed perturbation and decoding in pooled...Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing. A cis-splicing... New and notable Neurodegeneration Collection updates Last but not least, check out the new and noteworthy...

...typically contains iterons that serve as recognition sites for replication initiation proteins. These iterons...The plasmid does not have many restriction enzyme sites. Hosts often have a restriction barrier as a defense... al. 1977). So if a plasmid has few restriction sites, it may be able to sneak past this checkpoint.  ...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...inducibility (Toh et al. 2023), and additional cloning sites (Keen 1988).  Find broad host range recombinant ...

...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...transcriptional histories, accurate to within 40 minutes. Figure 4: Time-resolved protein ticker ...-PSD-95 [K28/43R] recently passed their initial tests with thick slice (70 μm) immunohistochemistry (IHC...fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection since we began...

...scientific mentor is typically a professor who operates a research group. Within that research group, ...mean everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks... in the next few days or weeks), look over your notes and protocol(s). Is there enough information there...throughout training, while others prefer to review their notes, process what they’ve just learned, and ask questions...for a copy of the protocol if you prefer to take notes on a printed protocol, or maybe you would like to...

...and dates of your actions. These records can take the form of written comments, added files, notes etc...Cancer Biology have shown similarly disappointing rates of reproducibility. These alarming results are due...the morning, to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything...everyday work to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based...

...binding of an aptamer to its target small molecule activates the fluorescence of the small molecule. Fluorescent...occurring regions in the 5’ UTR of mRNA that bind metabolites, leading to conformational changes that control... metabolite binding to the riboswitch instead activates Spinach fluorescence. In the search for something... to identify aptamers capable of inducing the brightest fluorescence (Filonov et al., 2014). Broccoli ...2017). Corn naturally dimerizes, and this dimer activates the fluorescence of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone...

...targets to measure! Let’s take a look at some of the hottest fluorescent biosensors new to Addgene this year...year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog is a new class of...smell the flowers - or rather, the lactate! The latest biosensor from Gary Yellen’s lab is LiLac, a fluorescence...fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato), they produce a temperature...over previous temperature indicators, it also demonstrates that taking advantage of fluorescence quenching...

... used to identify a gene mutation is the genetic test used for sickle cell anemia (2). Southern blotting...of restriction enzymes that cut DNA at specific sites (5). Imagine that you have a genome with unknown...cloning and analytical digests. A researcher can test the orientation of an insert in only a few hours...available although they are reported to have high error rates (10). It is projected that nanopore-based sequencing... C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia." New England Journal of ...

...recombination events, and the synthesis of alternative loxP sites to specify distinct patterns of recombination. In...Molecular and Cellular Biology, harnessed these attributes of Cre-lox to create the Brainbow mouse neural...defined using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions are mutually...be detected via immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine...doi: 10.1038/nrn2391. Epub 2008 Apr 30. PubMed. Intestinal crypt homeostasis results from neutral competition...

...with a red-shifted fluor. Here, NLuc internally excites the fluor through a process known as bioluminescent...exogenous excitation and with OgNLuc being the brightest since it had an emission near 600 nm – a clear...imaging. In all of these fusions coelenterazine intiates the fluorescent signal and these FP-RLuc8 fusions...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence...

...there is variable transduction of oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons...Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 Microglia AAV9 > AAV2 & AAV6 Astrocytes AAV8 > AAV2...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...

...curated their Ebola-related content. -And the latest updates on the ongoing outbreak from the CDC and WHO...Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective... data on five strains of Ebola representing the latest outbreak and earlier ones, along with links to ...

...References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced...up in mice as poorer performance on forced swim tests and less interest in sweets. Further examination...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during...

...double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame insertion of tag-encoding sequences ...assembling csRNPs in a 96-well plate format and tested parallel tag knock-in for 96 different genes in... negative control for the tagging experiment and test them in parallel so you have a good measure of ICC...clonal lines by single-cell deposition in 96-well plates: Characterize clonal lines by ICC and PCR genotyping...any cells positive for tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG...

...discovery. With these screens, the investigator generates a cell population where all genes are knocked ...groups and, as a consequence, which genes are good candidates for being involved in the studied phenotype. ...These targeted screens can allow a researcher to test which of the differentially expressed genes found... is added), and decide which genes you’d like to test for phenotypic effects. Typically the phenotype ...interest (that were not found by Green Listed), and test if any of the alternative names works better. To...

...costume, and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids,...table. This year, in addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made ...temperature and humidity favors fungal growth. The ants bites down and locks their jaws into a leaf, and dies ...Addgene See some of our previous Halloween costume contests ...

... (see schematic below), enabling a researcher to test multiple tags for one protein quickly and easily...easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...post-translational modifications added to proteins in other eukaryotes better than E. coli. Plasmids designed for these... systems may include a signal peptide (SP) that routes the protein through the secretory pathway to improve...

...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage... grants too with pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with...dinner and champagne in the lab. The Church Lab celebrates successful moments in the lab with parties in...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with ...