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We narrowed to 927 results for: TIM;

Showing: 381 - 400 of 927 results

  1. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the samples...AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718...

  2. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is ... (mostly), but can often achieve more structured time management conducive to work/life scheduling Diverse...to shed light on what paths to pursue. Spend some time really thinking about your motivations and what ...

  3. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...individuals for green cards because of the cost, long timeline and uncertainty associated with filing a permanent... a series of stages to refine and build skills overtime for success. Many people will have different roles... Ph.D. trained scientist. If the candidate is ultimately looking for a role in a different functional ...must be named in the media report (NPR, New York Times, respected online sources) and the media should ...must show that your company employs at least 3 full-time people engaged in researcher activities. The EB-...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...repository, they remain accessible during these times. Centralized reagent repositories, also known as...access to validated reagents, especially during a time when these materials may not be available from their...Addgene and has already been requested dozens of times in 2 weeks. This is a striking example of the contributions...Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol...

  5. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visit   Design and communicate a research plan Your time at the new research site is limited, and we all ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...research and teach you new techniques. Show up on time (don’t make your bench mentor wait on you!), make...amazing career opportunity, but also a really good time! If it sounds right for you, start thinking outside...

  6. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have been able to study the effects of various stimuli on global translation, but a visual technique to...by tracking the translation of a single mRNA over time. Both are helping researchers explore the complexity...that translates the mRNA. To permit tracking over time, Yan et al. devised a system that labels both mRNAs...fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites on ...

  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...Use the Viral Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your...from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back to...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...

  8. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...a high titer Cas9 lentivirus prep takes a bit of time and effort that, for many groups, may not be worthwhile...enough lentivirus for you to both perform an optimization-scale infection and at least one screening-scale... robust. What you don't need to do is spend your time troubleshooting reagent production, because that's...make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene,...

  9. Components of CRISPR/Cas9

    Type
    Blog Post
    ..., new Cas9 variants are being discovered all the time and the requirements of these different systems ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...direct repeat, combined with its adjacent spacer, ultimately encodes a single crRNA. The direct repeat regions...about all the different components of CRISPR, It's time to start thinking about how you can used them in...

  10. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... the hall, it is always a good idea to take some time to confirm that you are working with the correct...of all the plasmids we distribute. This method is time-intensive, so we recommend a variety of ways to ...enzyme. Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for...The dyes also run at predicted sizes so you can estimate how far down the gel your bands have traveled ...

  11. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...enhanced signal of eNL allows their use for the first time in single molecule imaging. Nano-lanterns and your... al. “Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.” Proc Natl Acad... variants of bright luminescent protein for real-time multicolour bioimaging.” Nat Commun. 7 (2016): 13718...Fluorescent Proteins Check Out Fluorescent Protein Timers Use Rosella to Study Autophagy Resources at Addgene.org...

  12. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often... other cellular functions and phenotypes. Since time is of the essence in clinical settings, looking ...that could enable future clinicians to improve real time decisions when rare variants are discovered in clinical...

  13. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...world of precision genome editing. When it comes time to (hopefully) validate successful mutation of your... we found these assays frustrating to work with, time consuming, and minimally informative. In this blog...Instead of tracking the reaction progress as in real time PCR, each reaction partition is individually assessed... duplexed primer probe assays often used in real time PCR. These assays can be designed to detect either...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...plants are being investigated on limited funding and time. While institutions that focus on creative problem...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change... 2014. Outside of the lab, Keira enjoys spending time with her husband and two Jack Russell Terriers. ...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...goals. These programs also serve as a great first-time mentoring experience. They make mentoring less mysterious... all group members to lead and learn at the same time Broadens the network of the participants Can be ...However, they find it awkward to ask someone for the time commitment of a formal mentoring relationship or...organizations as it brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar...

  16. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...some of their time. There are many professional coaches who charge for their advice and time. Consider this... or who your new contact will connect you to. Sometimes, it is not who you know but who knows about you...share their wisdom and they might just not have the time. Thank them anyway and move on. If they say yes,...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...cells (iPSCs) has been around for 10 years. In that time, scientists have used almost all available approaches...relatively simple to implement and doesn’t require time-consuming production of virus. In theory this is...efficiencies and they tend to express for longer periods of time than traditional plasmids due to lower activation...therapeutic applications, but it can take a long time to reprogram cells. In Kim et al, cells were exposed...rigorously assesses pluripotency, but they also take less time to complete. Morphology is a simple and fast to ...

  18. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...Benjamin Schwessinger, a postdoc at Berkeley at the time and an eLife ambassador, had previous experience... themselves were assessed and could improve over time. They achieved this by administering pre- and post-workshop...international team assembled the right people at the right time. Be inclusive and seek solutions by working with... industry partners, not the other way around. Sometimes, due to misconceptions and stereotypes, solving...

  19. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain is a tricky...localization, kinetics, and spectral properties, must be optimized simultaneously in order to generate a channelrhodopsin...reach the VTA. Mice were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering ...H134R) expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger...

  20. Summer SciComm: Preprints

    Type
    Blog Post
    ...of drawbacks, especially the increasingly growing time from submission to publication. It takes many months...peers in the field, and updated by the authors over time, providing a public record of changes, critiques...communication. Researchers often post a preprint at the same time as, or before, submitting the manuscript to a journal...your results, share them as best you can! At the time of writing, Mike Lacy was also employed by the American...
Showing: 381 - 400 of 927 results