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We narrowed to 936 results for: TIM;

Showing: 381 - 400 of 936 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...account for three years and have only tweeted 6 times: #fail. I’d hoped to use Twitter professionally ...scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top 20 Domains Tweeted by Scientists... contain scientific domains between 0-10% of the time; however, ~20% of life scientists  (biologists, ...ecologists) tweet scientific domains 20% or more of the time (red box). 8. Scientists tend to follow other scientists...

  3. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...took the time to answer our questions.   Advice for success in academia How can we shape our time in academia...passion. “Love what you are doing!” It helps when the time comes to work with full force. George Church 1....during their studies. They suggest that you make time for extracurricular activities both to develop new...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...exceeding a kit’s capacity can actually reduce your ultimate plasmid yield.  Take your average column-based...available for plasmid purification. Kits can be optimized based on the plasmid size, cell model, or overall...size. Some manufacturers offer kits specifically optimized for purification of these large plasmids, while...and for extracting RNA! (That’s a blog for another time, however!) Where can you look for help?  All manufacturers...Different Quantities, Different Uses Plasmids 101: Optimizing Plasmid Yields Bringing Sustainable Practices...

  5. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you...until the article is published. This policy gives us time to complete necessary quality control and legal ...distribution, and we’ve found that this step was sometimes unclear or missed altogether. Emailing Addgene...citation to the final journal article version any time later. We recognize that a preprint isn’t necessarily...

  6. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...with a master’s. At the time my degree felt like a consolation prize, but in time I realized its value. ...helped develop my science communication skills. Ultimately, my degree prepared me to be exactly the type...1. Are you interested in master’s-level jobs? Ultimately a degree is a means to gain employment, so it...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...an entire classification scheme, known as the Baltimore replication classes, has been developed around...around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...And now that you know what viral vectors are, it’s time to dive into how they’re used! We have a growing...

  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is ... (mostly), but can often achieve more structured time management conducive to work/life scheduling Diverse...to shed light on what paths to pursue. Spend some time really thinking about your motivations and what ...

  9. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...outstanding material transfer agreement (MTA) turnaround times: less than two days for material requests and less...agreement must be put in place. And subsequently, every time that material is requested, a material transfer ...By default, your TTO must sign an agreement each time a material is being requested or deposited. But,...future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to basically...

  10. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the samples...AAVs, it typically takes a few weeks to achieve optimal expression of XFPs. Any longer and the cells may...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016): 2718...

  11. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...individuals for green cards because of the cost, long timeline and uncertainty associated with filing a permanent... a series of stages to refine and build skills overtime for success. Many people will have different roles... Ph.D. trained scientist. If the candidate is ultimately looking for a role in a different functional ...must be named in the media report (NPR, New York Times, respected online sources) and the media should ...must show that your company employs at least 3 full-time people engaged in researcher activities. The EB-...

  12. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...visit   Design and communicate a research plan Your time at the new research site is limited, and we all ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...research and teach you new techniques. Show up on time (don’t make your bench mentor wait on you!), make...amazing career opportunity, but also a really good time! If it sounds right for you, start thinking outside...

  13. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...have been able to study the effects of various stimuli on global translation, but a visual technique to...by tracking the translation of a single mRNA over time. Both are helping researchers explore the complexity...that translates the mRNA. To permit tracking over time, Yan et al. devised a system that labels both mRNAs...fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites on ...

  14. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...Use the Viral Service Tips for 1st Time CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your...from 7.4 to 6.8). It’s possible that a longer vent time would be sufficient to return the sample back to...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...

  15. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...repository, they remain accessible during these times. Centralized reagent repositories, also known as...access to validated reagents, especially during a time when these materials may not be available from their...Addgene and has already been requested dozens of times in 2 weeks. This is a striking example of the contributions...Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...a high titer Cas9 lentivirus prep takes a bit of time and effort that, for many groups, may not be worthwhile...enough lentivirus for you to both perform an optimization-scale infection and at least one screening-scale... robust. What you don't need to do is spend your time troubleshooting reagent production, because that's...make new lentiviral preps on your own, fear not - anytime you order ready-to-use lentivirus from Addgene,...

  17. Components of CRISPR/Cas9

    Type
    Blog Post
    ..., new Cas9 variants are being discovered all the time and the requirements of these different systems ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...direct repeat, combined with its adjacent spacer, ultimately encodes a single crRNA. The direct repeat regions...about all the different components of CRISPR, It's time to start thinking about how you can used them in...

  18. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ... the hall, it is always a good idea to take some time to confirm that you are working with the correct...of all the plasmids we distribute. This method is time-intensive, so we recommend a variety of ways to ...enzyme. Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for...The dyes also run at predicted sizes so you can estimate how far down the gel your bands have traveled ...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...enhanced signal of eNL allows their use for the first time in single molecule imaging. Nano-lanterns and your... al. “Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.” Proc Natl Acad... variants of bright luminescent protein for real-time multicolour bioimaging.” Nat Commun. 7 (2016): 13718...Fluorescent Proteins Check Out Fluorescent Protein Timers Use Rosella to Study Autophagy Resources at Addgene.org...

  20. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... of these “variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we ...acid change based on maps of protein structure. Sometimes this provides a great deal of clarity. But, often... other cellular functions and phenotypes. Since time is of the essence in clinical settings, looking ...that could enable future clinicians to improve real time decisions when rare variants are discovered in clinical...
Showing: 381 - 400 of 936 results