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We narrowed to 1,076 results for: TOR

Showing: 381 - 400 of 1076 results

  1. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... for depositing labs to its website, allowing depositors to download their plasmid request data, as a ...information can be of personal interest to the depositor, but it can also be valuable data to share in ...total requests, total countries represented by requestors, year of first reagent sent, year with most reagents...is launched, it will show my data by default. Depositors can download their CSV file from Addgene by following...Dashboard After the upload, the name of the depositor and the date of downloading the CSV is shown (...and the source code is available in the Github repository (you can find the link in the upper right hand...dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim Goedhart is...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall... curves in their day-to-day work. Indeed, viral vector production facilities frequently use qPCR with ...with each step of the series diluted by the same factor. For example, Figure 1 depicts a 2-fold dilution... standard curves vary depending on a number of factors, including the user, equipment, assay incubation...measure.  Dynamic range depends on a number of factors, including the dilution series of the standard ...standard curve dilution series with lower dilution factors. If the unknown sample’s measurement is too high...assays you will be using ahead of time to see what factors may impact the dynamic range of the assay. Try ...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...including the internet!) can vary depending on factors like socioeconomic status and geographic location...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...restrictions (or both!) will be a major limiting factor at multiple points. Before you embark on a project... convey enough information to tell a compelling story in an image or do you need a full exhibit? In a ....  Static. Can be difficult to convey an entire story or context via still images. Some things cannot ...information. Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

  4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... the notation works? The + operator denotes intersection and the – operator denotes set difference. That...inclusive markers of regulatory regions in the genome, including enhancers, promoters, insulators, and more. I...proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ChIP-seq finds...of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as...enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that this matter exists, but...criteria: no TTTTT allowed (this is a polymerase terminator), and no off-target effects for the identified...

  5. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...us. Use a well working vector system This allows you to quickly assemble vectors and test different ideas...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...to quickly screen and investigate transcription factor candidates and the regulation of genes in the biosynthetic... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...system you can easily and quickly create customized vectors. For more information on MoClo for plants, check...

  6. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create... concrete happen. You may have more willing collaborators than you realize. Being at a university provides...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ... different countries). Here social media is a connector and virtual meeting place: You can follow interesting...as messages and keep you up-to-date on your collaborators’ professional developments without directly ...

  7. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Darrell Kotton is the director of the Center for Regenerative Medicine at Boston University Medical School... Darrell recently joined the Addgene Board of Directors where he hopes to help keep us focused on our ...Darrell's goals as a member of the Addgene board of directors - increasing open source sharing and helping us...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...

  8. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...they do so is through G protein-coupled receptors (GPCRs), receptors that play many roles in signaling processes...which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET is that the... between a luminescent donor and a fluorescent acceptor. TRUPATH uses an updated version of BRET called...constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...non-uniform across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze...Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... referred to as “vectors” or “constructs.” To insert a gene of interest into a vector, scientists may ...plasmid with its elements described below.   Vector Element Description Origin of Replication (ORI...the target gene. Vital component for expression vectors: determines which cell types the gene is expressed...Regardless, once the cloning steps are complete, the vector containing the newly inserted gene is transformed...Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment...about molecular cloning, how to choose a plasmid vector, molecular biology tools and references, and how...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...upon an indicator they developed and published in 2010 for visualizing the transcription factor cAMP-responsive...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple... variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors might now enable...

  11. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform... study has just been uploaded to the preprint repository BioRxiv. This example clearly illustrates the...  Figure 2: When a researcher needs a collaborator for a specific task, Crowdfight will: 1) Find...researchers, such as postdocs and principal investigators. They also come from many different scientific...website. Once curated by our in-house team of collaborators, the requests were forwarded to our volunteer...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

  12. Science Career Options

    Type
    Blog Post
    ...Scientific Illustrator Plant Breeder Zoological Worker Conservationist Documentary Director Parasitology...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor...Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer     Fieldwork...Bioinformatician Science Writer Grant Writer Journal Editor Science Marketer Software Developer Learn Science..., and how many jobs are normally available (as sectors such as forensics currently have too many applicants...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

  13. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...regulate botulinum neurotoxin were also created. The depositors demonstrated the utility of light-mediated suppression...slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids...acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These.... 2018. PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated ...microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression across...They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert site and a reporter...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...This will be highly dependent on vector dose. The color diversity in Vector Assisted Spectral Tracing (VAST...catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may...drop in transduction coverage compared to just one vector.   Q2: Do you think it is possible to make an efficient...this scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method...concentrated to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011...Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...Oleispira antarctica, and the P. haloplanktis Trigger Factor), that would allow the stable folding and expression...compared to the E. coli strain expressing the empty vector. And thus was born ELSA - our happy lil’ cold-tolerant...of stakeholders from industrial and commercial operators, government agencies, active researchers, as well...interact with personnel from the industry and brainstorm with each other. The event also featured a seminar...The talk highlighted the importance of reagent repositories and the myriad facilities offered by them which...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

  16. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Recently the Vellai lab has created an improved RNAi vector that leads to significantly stronger phenotypes...for mutant genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted...Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...Recently, the Sidhu group at the University of Toronto expanded the specificity landscape of the SH3 proteins...

  17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on any gene that they could...which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for...information from the structure to guide and improve activator design,” she said. “Before it was a little like...Konermann recognized as an ideal spot to fuse the activators instead of at the protein’s C terminus. With ...activation complex consisting of multiple distinct effector domains that work together synergistically, modeled...melanoma cells resistant to treatment with a BRAF inhibitor. (The test was the perfect complement for one ...repressing or inducing individual transcripts. The activator (CRISPRa) sgRNA library uses the sunCas9 system...

  18. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...viral vector samples were distributed in 2018 and we are poised for more offerings. The viral vector team...don’t have worry or wonder if providing a viral vector service was a good idea. The scientific community...don’t have to worry about getting support from depositors. We regularly hear how much they see the value... 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised to support our growth. Thanks...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...blog Subscribe to the Addgene blog Read our viral vector blog posts Learn more about Addgene Resources ...

  19. dTAG - You're it!

    Type
    Blog Post
    ...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest... target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological effects. ...et al., Nature Chemical Biology for examples). Monitor neo-substrates Small molecule degraders may recruit...and IZKF3 are cell-type specific transcription factors with important roles in Multiple Myeloma), it is...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...

  20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy... Mark Prescott. This system was developed for monitoring and analyzing autophagy of cytosol and organelles... samples treated with or without an autophagy inhibitor. While this method does provide a quantitative...the mammalian Atg8p homolog, can also be used to monitor autophagy, but under some conditions it aggregates...also possible to make Rosella fusions and thereby monitor autophagy of your protein of interest. Are you ...Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from Yeast to Human. Autophagy,3(3)...
Showing: 381 - 400 of 1076 results