...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps...

...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...

..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....about the science of race."     - Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes...

... a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track...

...your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple...You then run samples of your DNA at different dilutions and quantify based on band intensities of your...

... tap water. Flood the slide with Gram's iodine solution. Rinse briefly with tap water. Add ethyl alcohol...preparing the smear, and mixing the stains and solutions used, check out these resources.   Bruckner, ...

...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...

... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....

... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect...commonly called bad cholesterol). However, size distribution and concentration of ApoB-LPs has been shown...

...we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For...

...excited about the prospect of working toward a solution. At the end of the meeting, make sure to thank...Addgene Blog Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a ...

...close enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic... in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine...window. These systems are well-suited to directed evolution applications. Examples of these base editing systems...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...CAPTURE system, developed by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor... Kim, D., Kim, S., & Kim, J. (2018). Directed evolution of CRISPR-Cas9 to increase its specificity. Nature...

... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...PhDs in their careers with actionable steps and execution plans.   Resources  Resources for aspiring managers...

...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of...

... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ...

...gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target...

...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ...