We narrowed to 743 results for: UTY;
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TypeBlog Post... a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track...
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Cultivating Community Science at BosLab
TypeBlog Post...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post...your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple...You then run samples of your DNA at different dilutions and quantify based on band intensities of your... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect...commonly called bad cholesterol). However, size distribution and concentration of ApoB-LPs has been shown... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps... -
Celebrate National Book Month with Addgene
TypeBlog Post..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....about the science of race." - Joanne Kamens, Executive Director I Contain Multitudes by Ed Yong "Microbes... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender.... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post... tap water. Flood the slide with Gram's iodine solution. Rinse briefly with tap water. Add ethyl alcohol...preparing the smear, and mixing the stains and solutions used, check out these resources. Bruckner, ... -
DAP arrays for multiplex gene editing
TypeBlog Post...for a higher number of MPE. Therapeutic potential To explore the therapeutic potential of the DAP array... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post... untranslated mRNAs. The high specificity and resolution of this system are achieved by using multiple...analysis in vivo of translation with nucleotide resolution using ribosome profiling.” Science 324(5924) ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For... -
How to Lead a Great Meeting
TypeBlog Post...excited about the prospect of working toward a solution. At the end of the meeting, make sure to thank...Addgene Blog Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a ... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post... "do it yourself" can result in burnout and underutilized team potential. I often thought that performing...PhDs in their careers with actionable steps and execution plans. Resources Resources for aspiring managers... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...more effectively used in a technology or as a therapeutic. A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ... -
Viral Vectors 101: Types of viruses
TypeBlog Post...gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...