...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...

...these reporters, editing typically results in a change in the fluorescent readout. In this blog we will...for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells.  A...GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did not surpass...

...related plasmids in our collection, we’ve relied on Sanger sequencing as a first pass QC. The lab team manually...that process is complete, QC scientists prepare Sanger reactions by mixing this isolated plasmid DNA with...sequencing primers, and hand deliver them to our Sanger provider. These results come back within 8-12 hours...

...bacteria out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which... of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures,...discussions. Not only are such endeavors a welcome changeover from conventional academic settings, these are...

...enzymes function across a much broader temperature range than BioID, which only functions optimally at 37... Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks Article contributed...equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment or ...

...microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol....target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error...

...heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation...step would be to validate your new construct by Sanger sequence analysis. But for this research, it was...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...

...overlaps with the XbaI cut site (depicted as an orange line) because the restriction site is followed ...mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme will cut...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

...upon themselves to teach me Python, a programming language favored by biologists. Almost as soon as I began...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...

...paper, Yinong Yang’s group did just that as part of their CRISPR work in rice plants. Yang was intrigued...endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11)...

....  Here are some tips to get out of the rut of hanging out with only your lab mates (let’s face it, you... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...better than current technology and get customers to change behavior. When you are ready to commit to the company...

... in D.C. for a few years, Susan was ready for a change of scenery. She moved to Seattle and continued ... there there’s always plenty of writing: plain-language stories about Fred Hutch research, pieces for ... scientist or someone who’s affected by climate change or affected by a superbug. I think we solve these...

...microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody conjugation...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based... choice for ELISAs.  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible...

...nutrient and ionic balance of the cells,” says Dr Sangeetha Iyer, ACD’s Scientific Advisor. It’s possible ...Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly ... Jennifer Tsang...

...split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted...

...can be hard to visualize or perturb these slower-changing bioelectric states in living cells. Since bioelectrical...PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction protein-mediated cues control... Angela Abitua...

...plasmids modified from pSC101 with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential... Jennifer Tsang...

...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips... simplify the process. First, nearly all of our Sanger sequencing is done from glycerol stocks (overnight..., and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make sure to...

... loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called... Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the Joung lab at Harvard...