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We narrowed to 492 results for: ara-2

Showing: 381 - 400 of 492 results

  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into... “designer” channelrhodopsins with desired characteristics. The model was trained to predict if a channelrhodopsin...these remaining sequences were selected for characterization with patch clamp electrophysiology. All thirty...named ChRger1, ChRger2, and ChRger3, were then characterized further by expression in cultured neurons and...performed well in a tissue culture dish, that doesn’t guarantee their success in vivo. Therefore, the Arnold and...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    ...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method...gradients and ultracentrifuged for up to 20 hours to separate the intact RNA from DNA and proteins. Although...and Sacchi showed that RNA could be effectively separated from DNA and proteins by a simple extraction protocol...thiocyanate solution. However, instead of RNA separation using cesium-chloride gradients, water-saturated...ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in the top, aqueous layer...

  4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...main decisions to make: 1. Which modification? and 2. Which viral vector should be used? Modifying gene...need to be given some time to adapt to a testing apparatus. General stress levels of the test animal may ...the humans’ audible spectrum. The experimental apparatus should be cleaned thoroughly between animals as...beginning. Variability source Standardization parameters Animal Strain, age, sex, housing conditions...order to maximize possible effects. Injection Parameters: The injection needle should be as thin as possible...

  5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ..., but at a lower efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple... other dNTPs. Once the vector and insert are (separately) digested to create 10–12 base “chewed back” ... T4 polymerase (left branch); by annealing two separate PCR products (middle branch); or by incomplete...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... and transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above...for processing several sequencing reactions in parallel. We have a few tricks to simplify the process....saves us the step of purifying the DNA and gives comparable success rates and sequence quality, which is ...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...10.3389/fmicb.2020.607028  Riley, R., et al., Comparative genomics of biotechnologically important yeasts...

  9. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Without binding to the guide RNA, Cas9 cannot cut. 2. Bind to target DNA in the presence of a guide RNA...bacterial genomes consist of repeated elements separated by unique sequences. When researchers first discovered...

  10. How Addgene Creates Content

    Type
    Blog Post
    ...and in line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer...the antibody guide always gets an expert review, separate from the author, and a second review from someone...

  11. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant...and validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a ...format. Figure 6: Add sequence, maps, and files. Step 2: Gene and Insert *Note: This page will not appear ... into it. You will have the option to indicate separate promoters, tags, or cloning information for each...

  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...

  14. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral...if preparing several different lentiviruses in parallel, this approach should be considered. Similar to...consider using a reverse transcriptase mutant in a separate control reaction. This will allow you to quantify...

  15. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...book.  Many thanks to our guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different... than split-sfCherry3. It was created by Maria Ingaramo, Jerome Goudeau, Cynthia Kenyon, and colleagues...Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library can be used... used to identify genetic interactions between paralogs. The exon-deletion hybrid gRNA library deletes...

  17. CRISPR in the Clinic

    Type
    Blog Post
    ... Cure for HIV/AIDS. Int J Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org... therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a gene known...known as Crygc (Wu et al). They found that the cataracts phenotype could be rescued by zygote co-injection...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... to remove this default setting. Timesaving Tip #2: blastx searches are inherently slower than blastn... BLAST button, consider the Program Selection parameter, as this will affect the amount of time to perform...BLAST button, you may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST...have to remember to adjust any blastp algorithm parameters. We use the default scoring matrix BLOSUM62, ...

  19. Science Guides

    Type
    Guide
    ...specific control of activity. Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic...Pooled Libraries Pooled libraries are single preparations of many different plasmids. Plasmids within ...

  20. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig.... 2011 Jun;3(6):677-713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview...
Showing: 381 - 400 of 492 results