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We narrowed to 951 results for: cha

Showing: 381 - 400 of 951 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...researchers working on rare diseases, offering them the chance to win some of the latest tools and technologies...They now have some theories on the underlying mechanism, involving the primary cilia. They’ll put a variety...number of stressors, including emotional stress or changes in temperature.  “The disease was first described...a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers...syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called...

  2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright...mutagenesis approach and found that specific amino acid changes favored blue-shifting more than others. During ...Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging."...blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out...

  3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power so the samples... voltmeter on top of the battery box to monitor charge status and a disconnect switch between the battery...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples... Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S rRNA gene sequencing which...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 ...

  4. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...master’s thesis in science communication, I had the chance to carry out a comprehensive review of the literature...findings relevant to my background (neuroimaging, biomechanics, neuropsychology) and from areas in which I ... and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing effort...display in our building, each day brushing past and chatting with individuals whose research ranged from wildlife...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation ...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as...reliably locate your injection site. We recently purchased this model ( no longer available through Kopf)...editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter 1:Unit 1 20 1-18. doi: 10.1002/0471142301.ns0120s59...PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...Fluorophore detection Live APC-Cy7 channel eGFP FITC channel CD45 BV421   Figure...of fluorophores from Figure 1. For example, the chart tells us that PE and PE-Cy7 are "very bright" when...conjugated to Cy7, however, (APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that ...fluorophore, we have to tell the cytometer which channel to use to detect eGFP. As you saw in Figure 4, ...overlaps with FITC, hence reading out the FITC channel in the cytometer's software (without using the ...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... to a channel number based on intensity. Higher intensity events are assigned to higher channels. In a...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...of the protein. In some cases this can lead to changes or a complete loss of protein function. In addition...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...stream oscillates to generate droplets that are charged as they pass through a metal deflection plate. ...

  8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen...her group are interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance ... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... investigates the molecular and cellular etiopathomechanisms of ischemic stroke, the most common type ... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury....

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...transcriptional changes. Using this road map, and what was known about previously characterized TFs, the Zhang...association of the uncharacterized TFs is a useful finding. Transcriptional program changes from this screen...can be used with other selection and screening mechanisms, although the unique barcodes for each TF help...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging period...Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16,...give back by supporting local businesses (ex: purchasing a gift card to use later or ordering take-out...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

  11. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the transduced...is to increase the AAV concentration. In this flowchart, you can see the dwindling proportion of AAV particles...gets transported up the cell’s axon. While the mechanism for this function is not yet fully understood,...S9-S15. PubMed PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...results. GBEs seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly... A's, producing C → A edits. Figure 3: Mechanism of cytosine transversion editors. Red arrows indicate...agreed on the first part of the likely editing mechanism: cytidine deaminase converts C to U, and UNG excises... Base Editing terms   Base transition Base changes between purines (A ↔ G) or between pyrimidines ...pyrimidines (C ↔ T).   Base transversion  Base changes from purine (A or G) to pyrimidine (C or T) or vice-versa...Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... virus used. All of these expression control mechanisms require a specific modification of the viral ... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...Viral vectors are being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the...

  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...open-source microscopy seeks to change this. This recent push gives users the chance to understand the "black...much more involved. Challenges such as the provision of an accessible supply chain for parts that are ...Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative ...

  16. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... the materials in our catalog are very well-characterized. During this time, we’ve also created a variety...this, throughout the year we rolled out multiple changes to our website, including the recent homepage and... a look at the least visible part behind these changes: the backend technology that powers the new website...granularly customize the search engine, and a scoping mechanism that allows category-specific, narrow searches...among others. We also created a normalization mechanism to “collapse” variations and synonyms of the same...

  17. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....Optogenetic control of protein binding using light-switchable nanobodies." BioRxiv (2019): 739201.  Additional...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...and has provided clues to their previously uncharacterized cellular roles. In the current phase of NURSA... expression and reporter plasmids and the free exchange of these vectors between laboratories. The development...example, was a key development in the functional characterization of NRs and coregulators, and although the ...Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular and Cellular Biology...Nuclear Receptor Signaling.   Further Reading: Chawla, A., Repa, J. J., Evans, R. M.and Mangelsdorf, ...

  19. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...person in the group for your needs. Beth Schachter of Beth Schachter Consulting is part of that group and ...are for the coaching relationship. Do you want to change jobs? Grow your professional presence? Learn a ...and make direct suggestions for improvement and change. Work with the coach to define how you will know... Polk of From PhD to Life. She runs great tweet chats, has an outstanding blog and, while her PhD is in...

  20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...go ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions...Continuing efforts to gather information and expedite change We are continuing to update our FLSA and Postdocs..., under “How institutional plans have/have not changed since the injunction”. Postdocs around the country...enterprise and promote their voices in driving changes in science. Follow him on Twitter @BiophysicalFrog...the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read ...
Showing: 381 - 400 of 951 results