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We narrowed to 876 results for: lic

Showing: 381 - 400 of 876 results

  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor...PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed...PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA... PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...aware that the fluorescent proteins reported in publications are only characterized under a limited number...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation...

  4. Antibody Production

    Type
    Collection
    ...cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ... protein interactions (Alice Ting Lab 2020) Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-...Image modified from Feng et al. 2017 under CC-BY license. Many split-FP fragments are named according...Split-Fluorescent Protein Tags Browse the table below, and click the Article link to find all the plasmids from the...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...for more complicated plasmids, like those that contain synthesized regions that have no publicly available...often find a few mismatches in the origin of replication or other common backbone elements. Since we’ve...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...prevent the formation of replication competent lentivirus (RCL). Replication competence can arise from...end-users, size of the transgene, and downstream applications should all be considered when choosing the correct... as resources available, cost and downstream applications. For example, while the PEG precipitation method...

  9. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments...Phagocata gracillis, the process is a little more complicated. These strains are more picky eaters and refuse...easily investigate in planarians. References 1. Alice Accorsi, Monique M. Williams, Eric J. Ross, Sofia...

  10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ... become very different due to a whole genome duplication and extensive rearrangements in the S. cerevisiae...overexpression vector. This plasmid contains origins of replication and selectable markers that allow students to...functional MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes...undergraduate courses and in communicating science to the public. You can find out more about Clare at the Biology...

  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... as a helicase. Following recruitment by Cascade, Cas3 nicks the non-target strand. The helicase domain...After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously, by continuously...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  12. Transferable Skills: Negotiation

    Type
    Blog Post
    ... feeling of conflict many of us associate with negotiating (check out more about conflict resolution)....examples of negotiation have been: A job offer conflicts with a pre-planned vacation I’m looking forward...blog More from the transferable skills guide: Conflict Resolution Time management Managing a team Leadership...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...setting—especially if job applications require teaching experience or high numbers of publications, which may take...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... proprietary compounds or information. Where publication is expected, the process is similar to academia...

  15. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...T4 DNA ligase. Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in...efficiency measurements using similarly sized Lambda amplicon inserts to control for any suppression of background...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ... in learning more about any one of them, please click on the links throughout the text or see the “Additional...cloning. Sequence and Ligation Independent Cloning (SLIC) could also be a great choice, because the T4 polymerase...The ligation-independent methods like Gibson and SLIC are also quite efficient; although efficiency often...some of the homology based methods, like Gibson or SLIC, since one can pool the components and define their...

  17. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... millions of people die from cancer-related complications each year. Despite these grim statistics, new...non-cancerous cells, such as those within hair follicles and lining the digestive system, causing discomfort...any of these genes through several different applications such as fluorescent protein fusion studies and...of interest to their laboratory. A single mouse click on a gene of interest will direct the investigator...

  18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...

  19. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...with an abundance of protocols and examples of applications from across disciplines now available. In this...experiment or evaluating ExM images in a new publication.         Why Expansion Microscopy?  Traditional...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...

  20. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests ...14%). Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month...fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student at Harvard...
Showing: 381 - 400 of 876 results