Addgene

We narrowed to 502 results for: trac

Showing: 381 - 400 of 502 results

Results per page:

CRISPR 101: Cas9 vs. The Other Cas(s)

Type

Blog Post

...about a third of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The...
Summer SciComm Series: Cognitive Load

Type

Blog Post

... the information. Maybe I needed to remove a distraction, such as pausing or turning off the slide deck...
Antibodies 101: Affinity Reagents

Type

Blog Post

...eukaryotic cells, allowing for them to be used as intracellular inhibitors. Aptamers are not proteins at all...
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Type

Blog Post

... human cell line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay...
Transferable Skills Guide: Cross-team Communication

Type

Blog Post

...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...
Gendered Innovations: Why Does Sex of the Cell Matter?

Type

Blog Post

... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...
The Future of Research Symposium Boston 2015

Type

Blog Post

..., less than 15% of postdocs had achieved tenure-track positions within 5-7 years of earning their PhDs...
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Type

Blog Post

... the action. If the paper is well-written, the abstract should give you a sense of the importance of the...
Career Coaching for Scientists: Why and Where Do I Find One?

Type

Blog Post

... certifications? How can they demonstrate their track record? Look for a coach that specializes in scientist...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells ...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...these constructs, developmental biologists have to extract RNA from their organism of interest, use the RNA...
Lab Automation at Addgene

Type

Blog Post

...from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find protocols...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

...for all protein coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008). ...
Plasmids 101: Modular Cloning

Type

Blog Post

...lot of optimization - which is why MoClo is so attractive! It allows researchers to assemble and test many...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

...Pages Other Links Check out Pawluk et al's Video Abstract Read up on All Things CRISPR with These Papers...
Viral Vectors 101: Chemogenetics

Type

Blog Post

...nonnative ligands. However, they retain their intracellular interactions and still set off the same pathways...
Viral Vectors 101: Virus Safety

Type

Blog Post

... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...

Showing: 381 - 400 of 502 results

Results per page:

CRISPR 101: Cas9 vs. The Other Cas(s)

Summer SciComm Series: Cognitive Load

Antibodies 101: Affinity Reagents

Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

Transferable Skills Guide: Cross-team Communication

Gendered Innovations: Why Does Sex of the Cell Matter?

The Future of Research Symposium Boston 2015

Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Career Coaching for Scientists: Why and Where Do I Find One?

Fluorescent Proteins 101: Introduction to FRET

Plasmids for Endogenous Gene Tagging in Human Cells

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Educational Planarian Tools for Teaching Developmental Biology Techniques

Lab Automation at Addgene

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Open Resources and Plasmid Tools For Studying C. elegans

Plasmids 101: Modular Cloning

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Viral Vectors 101: Chemogenetics

Viral Vectors 101: Virus Safety

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections