We narrowed to 536 results for: val

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...nucleofection as well as the sequences of our validated editing reagents can be found on our plasmid pages...

2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...interests. You can look at something as basic as survival. For those interested in development, organs of...

3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the number, the higher the ...

4. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ...thus providing a starting point for downstream validation experiments. But, there have been two huge challenges...

5. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   Blog Post

   ...need to make a change Provide perspective on core values at another company/department Give more candid ...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   ...shifts in expression, using a histogram plot and MFI values to characterize your samples may allow for better...

7. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   Blog Post

   ...bringing a systematic and disciplined approach to evaluating and improving the effectiveness of the Plan's...

8. Hot Plasmids - May 2022

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   Blog Post

   ...plex with two barcodes per cell. The researchers validated their work with the “signalome,” a 12-plex mixed-subclone...

9. Hot Plasmids - February 2022

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   Blog Post

   ...Cas12a. Image from DeWeirdt, et al. 2021 To validate this optimized genetic screening technique, researchers...

10. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ...Because of the steps involved in producing and validating monoclonal antibodies, their cost is higher than...

11. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...easily sort them by other parameters, such as E-value for protein sequence searches. Additionally, the...

12. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

    ...provide resources, knowledge, and most importantly, validated protocols (using readily available reagents!) ...

13. Viral Vectors 101: Types of viruses

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    Blog Post

    ...evolutionarily optimized gene delivery devices. Their survival depends on their ability to deliver and permanently...

14. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...interpretation of results. Take extra care when evaluating how this serotype works in your system. Chemogenetic...

15. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

    ...for analyzing time-lapse images, quantitatively evaluating antibody staining, or counting cells are now ...

16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    ...named GEMCherry2, that can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al...

17. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize that growth ...

18. Behind the scenes: Addgene’s new search engine and more

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    Blog Post

    ...term that appear in our data into a single filter value. For example, there is only one viral prep filter...

19. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    ...Gryder. Addgene depositors or not, your time is valuable ...

20. Plasmids 101: Inducible Promoters

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    Blog Post

    ...inducible pLac promoter. This promoter requires removal of the lac repressor (lacI protein) for transcription...