We narrowed to 911 results for: TRO

... fibers are drawn with microelectronics included in them for downstream control – this is important later...neural fibers developed by the Anikeeva lab are microelectronic fibers made from thermoplastic polymers that...optogenetic purposes, measure temperature, study electrophysiology, and deliver small molecules.   Fun fact:...(Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates optical stimulation...fibers as previously described, then measured electrophysiological potential afterwards. This measurement trended..., P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable Neuromodulation... Anikeeva, P. (2019). Flexible fiber-based optoelectronics for neural interfaces. Chemical Society Reviews...

...intersectional studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs ...our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium ...scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...used in combination with Cre for intersectional control of specific neuronal populations. We now offer ... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ...

.... Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret...many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for ...mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control offered by the...for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype testing...also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated,... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO...

...Cell-surface protein labeling tools for neurons and astrocytes Nerve-to-cancer cell mitochondrial transfer tracker...classrooms Cell-surface proteomics for neurons and astrocytes By Mike Lacy Cell-cell interactions underlie ...important in the brain. To better understand how astrocytes and neurons interact, the labs of Baljit Khakh...extracellularly-anchored horseradish peroxidase (HRP) in striatal astrocytes and neurons to label surface proteins with a ...Biotinylated proteins were isolated, identified by mass spectrometry, and classified by source cell based on single-cell...cell type, the cell-surface shared proteome of astrocytes and neurons (CS SPAN) featured extracellular ... for future studies of the molecular bases of astrocyte-neuron interactions. Plus, the tools used here...

...type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein, the authors...elavl3/HuC), microglial/macrophage (mpeg1.1), and astrocytic (gfap).This toolbox adds new neuronal tools to...to express the transcription factors from a polycistronic cassette. However, it was unknown whether variations...variations in gene expression from these polycistronic cassettes could lead to comparable experimental...Woltjen Lab compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...reprogramming and global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had...

... and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing the ...individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9 technology... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving...the tag through homology directed repair To introduce the fluorescent tag to the cells, we used CRISPR/Cas9...with the cut, we trick the cell into using the introduced donor plasmid as a repair template. This results...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...Figure 3: Inducing the double strand break and introducing the tag. Created with BioRender.com. Plasmids...

...about the tissue tropism of these and other AAVs. Figure 4: AAV6 and AAV11 tropism and applications...ISYmu1 and an ωRNA under the same promoter, they introduced heritable genome edits in a single step with ...clonal dynamics during differentiation after they introduced mutations that drive leukemia (Figure 3). ... Why these serotypes? AAV6 offers broad tissue tropism for gene therapy and ex vivo cell engineering applications...administration. AAV11 can transduce neurons for retrograde labeling, making it a powerful tool for circuit...applications. A) AAV6 is reported to have broad tissue tropism, including skeletal and cardiac muscle, airway ... studies have shown that AAV11 enables potent retrograde labeling of projection neurons. Created with ...

...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...and SbcCD nucleases from digesting linear DNA introduced into the E. coli. Exo: Exo is a 5’→3’ dsDNA-dependent...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....dsDNA recombineering substrates to avoid errors introduced during PCR. ssDNA Substrate ssDNA substrates ...that avoid MMR activation.  The first way is to introduce a C/C mismatch at or within 6 base pairs of the...1% or 25-50% when activation of MMR is avoided Introduce C/C mismatches or silent changes in wobble codons...these will be discussed in more detail below).  Controlling expression of Red proteins is critical for minimizing...

...would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...guide’s recognition sequence is destroyed via mutation. By introducing a mutation in either the PAM or...Identify where in the genome you would like to introduce your knock-in. Then choose which Cas enzyme to...region of a gene, make sure that the PAM edit you introduce is a silent mutation, so you don’t accidentally...to your culture media prior to and after Cas9 introduction increases HDR, just make sure to remove the ...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLIFE. (2014)...efficient homology-directed repair and termperature-controlled genome editing. Nat. Com., 8(2024), (2017). 10.1038...

...emission Before we design your first panel, we will introduce some background on the color spectrum and the ... photons are absorbed by matter, they promote electrons within atoms to a higher energy state. This process... called excitation. After a short period, the electrons revert to a lower energy state, whereby a photon...those of absorbed photons. When photons excite electrons of a fluorophore, they can do so at a range of...by the cytometer. Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few...absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   Figure 2: Excitation... real-life advantage of a brighter fluorophore? Strong brightness makes it easier to distinguish a signal...