We narrowed to 470 results for: Mag;
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TypeBlog Post...amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed PMID: 25307933...
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CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... in a single flask. Through a bit of stochastic magic and Poisson statistics, each sub-population has,... -
CRISPR Antimicrobials
TypeBlog Post... resensitization, or may also cause cell death. Image from Beisel et al., licensed under a Creative Commons... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...within a plate that can hold up to 96 tubes (see image below). Each plate has numbers and letters to give... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...hundreds of plasmid requests out each day. As you may imagine, it keeps all of us quite busy. Though there still... -
Plasmids 101: Protein tags
TypeBlog Post... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...popular Chinese social media platforms. As you can imagine, shipping to so many countries means that our shipping... -
Viral Production at Addgene
TypeBlog Post...corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced... -
How to Be an Excellent Trainee
TypeBlog Post...experiments. Unlike in lab courses, where everything is magically made up by helpful TAs or assistants, and sitting... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post.... Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...optimization of fluorescent proteins, and live cell imaging approaches. Erik is also an avid long distance ... -
Lab to Office Culture Shock
TypeBlog Post...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench... -
CRISPR-mediated Plant Base Editors
TypeBlog Post... Scholar at the Pennsylvania State University. Imagine you are dealing with a defective gene, Xm, the ... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020. Broad tropism: ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...enthusiasts to start a microscopy seminar to connect the imaging community and to create a platform for discussions... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...PMID: 9618448 Diaz R, Rech J, Bouet J-Y (2015) Imaging centromere-based incompatibilities: Insights into... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Singleton. Figure 1: The Binning Singletons logo. Image: James et al., 2020. Help me stranger - Finding... -
Career Insights: Technical Support Specialist
TypeBlog Post...Many thanks to Eric Perkins and Stephanie Hays for images used in this post. Klaus Wanisch is an Outreach...