We narrowed to 989 results for: IGH@

...developed, which differ in the number, stability, and brightness of the fluorescent proteins used in the system...Brainbow 3.0 (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2...system to boost XFP expression. The increased brightness of Tetbow allows this system to be used to trace...Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent...

...overview of plant genome engineering using CRISPR, highlight the specific modifications to CRISPR machinery...Lab.  As with all plasmids in the repository, we highly recommend reading the associated publications or...10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing using virally...

...to make them serve you well. The happy byproduct might just be a career opportunity. The focus of this...content. Create a Google.slides or Powerpoint file right now to capture interesting key results, striking...need to define a term, but are worried that this might sound condescending, consider explaining the term...Additional Resources Joanne's "How to Choose the Right Next Lab or Workplace" On YouTube Joanne's 10 Things...

...Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein modification...photoreactive arylazide group. Under strong visible light, the arylazide group becomes reactive and forms ...: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of Proteins.” Methods Mol...PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules on...

...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...provided you have some idea of where in the genome you might expect off target editing to occur. Luckily, in ...you to Sanger sequence a manageable number of the higher probability locations. If you’re worried about ...Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target...

... from a default OFF to an ON state, and how you might use these in your research. Today, we’ll look at...Take a look at all of the options and decide what might work best for your experiments! This post was originally...References and Resources References Gossen M, Bujard H. Tight control of gene expression in mammalian cells by...PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator...

... site.  T-RL= transposon right-left orientation. T-LR= transposons left-right orientation. Image from ...2009). hySB100x improves on SB100X by having 30% higher transposition activity (Voigt et al., 2016). Sleeping...sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however, efficiently...

...-radius":"10px","padding-left":"255px","padding-right":"255px"}{% endraw %}{% end_module_attribute %}{...important that your gRNA sequence is designed to be highly active and reduce off targets. For CRISPRa and ...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires...genetic screens using genome-wide libraries have shown high concordance between different sequences targeting...

...backwards. To publish a submitted manuscript in a high-profile journal typically takes 6-12 months depending...technology may not exist to answer the question or it might be extremely difficult for you to get the needed...for a summer undergrad, rotating grad student or high school student. Learn to teach how to design, perform...improves your perspective like intense exercise. I highly recommend running or biking or something that demands...

...activated, the protein emits a brief yet bright burst of light, visible to the naked eye, at regular intervals...forever change the fly lab experience. The Frequency Light Interval System, or FLIS, incorporates a bioluminescence...

...Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork...trophy among 6 people, but you’ve earned bragging rights for the next 12 months. Your team’s creativity,... favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite team costume...

...little screening for DNA markers like the presence of high risk (HR) Human Papillomavirus (HPV). As Gladys ...individuals who are infected with HR HPV and therefore at high risk for cervical cancer development and also better...disease severity. Individuals who are shown to be at high risk by these screens can then undergo more routine...

...service pAAV_hSyn1_nLightG AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors...of synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use...

...targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ends of the branches...branches contain the antigen binding sites and are highly variable, allowing exquisite specificity. This ...rabbit antibodies that exhibit increased avidity to high profile antigens; and 4) generating mAbs against...

...accelerate research and discovery by improving access to high-quality scientific materials and information. Our... repository of valuable research materials that might otherwise be lost amid lab closures or project terminations...Providing essential services: We continue to offer high-quality DNA sequencing, viral vector preparations...

...only two options, but very little data on which might work in your application. It can be quite difficult...materials and methods section. This section will vary slightly depending on the application — flow cytometry,...Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

... a few easy and fast things you can start doing right away to reduce your consumption in the lab: Turn...your oven on all day to save time making dinner at night, so why leave your lab equipment on? This applies...fact, some scientists speculate the -80 °C setting might have been a marketing campaign! MIT Green Labs has...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....recognizes a simple dinucleotide PAM (N4CC), lending to high target site density (average every 8 bp). Nme2Cas9...

...look and feel of addgene.org. Some you’ll notice right away, while others may be less obvious — but every...you can use the links under the search box to go straight to your materials of interest!   Figure 1: The...bar New menu Most common activities can be done straight from the new search bar, but the menu just above...

... also selected for other key properties, notably high solubility, limited affinity for other proteins,...interactions, particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will...Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian...

... (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight). If ...clean reliable DNA sequence Anything less and you might suspect contamination in your sample or consider...

...physicists, chemists, and mathematicians. We hope to highlight every science field as we expand and encourage... This would help you direct your content to the right audience. Avoid using scientific jargons. Someone...

...pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000 views ...researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....identified 67 genera of Proteobacteria that this toolkit might be used in. Read the paper in ACS Synthetic Biology...

...science accessible in one way or another to those who might be in a position to build on that work. That’s how...to consider where the open access (OA) movement might be going is to consider some of the places it’s ...providers and participation from many well-respected and high-profile universities. We particularly recommend ...

...functionally validating the thousands of mutations that might play a role in the progression of cancer are often...mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using data from The..., they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (...

...directory is crucial because it's hard to find the right phage for the job. Phages are viruses that can infect... have someone that really needs it, but then it might to be too late,” Sacher says. As the labs in the...sample is limited, only the labs that have the highest potential to possess the correct phages will test...

...excitation at 488 nm, photoconverted with 405 nm light, and then captured in the red state after excitation...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have...Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein...

...within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists across...with different technical knowledge that could help fight COVID-19. They’ve also collated a list of useful...  "We realized that there is now a huge pool of highly skilled scientists, willing to volunteer their ...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters...