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We narrowed to 928 results for: INA

Showing: 601 - 630 of 928 results

  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely...develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s a natural .... "Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly important...find my tips for a perfect science talk here). Finally, my main content organizer is the amazing app Evernote...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...marketing, commercialization, collaborative, and financial functions associated with the business. Commercial...business, in healthcare economics, or in some combination of these fields. I got my PhD a couple of years...trends and workings of the healthcare industry. Finally, on-campus resources like career offices, classes...

  4. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to induce gene...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes...JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    ... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...sequences each week. Our internal software was originally designed to help us work in large formats, such...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...concentration.  Screening time Phew! You are now (finally!) ready to use your transduced cells to perform...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating as they are good at...Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. ...

  11. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...Created with BioRender.com. In recent years, deaminase-mediated base editing (cytosine base editor or...FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...sequence, people often use terminology like “C-terminal fusion,” but I find this very confusing because... into the POI can work well since the N- and C-terminal residues of the fluorescent protein are relatively...assess whether the chimera is still functional. Final words on fluorescent protein fusions There are several...

  14. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...the literature was reproducible by their groups. Finally results from the Reproducibility Project: Cancer...to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything is covered...significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting, and the ...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration...have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle When IDLVs... can be generated through either homologous recombination or non-homologous end joining (see Figure 2)...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...blotting, a primary epitope-targeting antibody can originate from a range of species; for example, rabbit or...include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin ligases; and many ...  More resources on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide...

  17. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...substantive (“Person C told Person A one thing and inadvertently told Person B something else.") The majority...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...from the B cell antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s ...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

  19. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...

  20. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking...their level of explanation in presenting data. Finally, let me take this opportunity to recommend all ...your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities to show cool visuals...

  21. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality... designed multiple gRNAs to target the 5’ long terminal repeat (LTR) of the HIV-1 genome, which acts as... Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells ...

  22. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this...thought to be reversible once the effector is inactivated/removed from the system. In contrast, epigenetic...

  23. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to...repair to allow for detection, not Homologous Recombination or Alternative End Joining, two alternative ...different gRNA would have distinct off-target sites, eliminating its effectiveness as a control. Since off-targets...

  24. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...transformation due to background re-circularization and contamination from uncut plasmid. 4. Transformation Transform... higher competency cells. Additionally, if your final product is going to be very large (>10kb) you might...DNA and lets other materials pass through; and, finally, selectively elute the plasmid DNA from the column...

  25. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...

  26. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...be analyzed with a paired t-test, because they eliminate subject-to-subject variation (the test assesses...tests exist to identify significant pairwise combinations – Tukey’s test, Newman-Keuls, Duncan’s multiple...Comparison of Multivariate Means across Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study...

  27. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...

  29. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

  30. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ... and quantify how new technologies are being disseminated through the scientific community. Data on CRISPR...technology was also greatly accelerated through a combination of open-source distributors such as Addgene, ...
Showing: 601 - 630 of 928 results