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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 922 results for: POR

Showing: 601 - 630 of 922 results

  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

  6. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

  9. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

  10. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  21. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...recommends that you sequence any portion of the plasmid that is important for your experiments. There are... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence the entire...an example of a trace file from a high-quality portion of a sequencing reaction: Sequence near the beginning...unreliable. This is an example of a trace file from a portion of a sequencing reaction with high background: ...

  22. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

  25. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  26. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  27. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  29. Components of CRISPR/Cas9

    Type
    Blog Post
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  30. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...
Showing: 601 - 630 of 922 results