We narrowed to 730 results for: ACE;
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TypeBlog Post...communication, be it in your academic lab or your workplace, is an ongoing process. It is never really done...you try any of these approaches in your lab or workplace, let me know. If you have come upon any other ...
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A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...SARS-CoV-2 virus depends on its spike protein to bind ACE2, the host cellular receptor. To more easily study...SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV. The lab... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post... to undergraduate students (1, 2), but educators face significant challenges in designing suitable projects...complementation should only occur when galactose replaces glucose as the carbon source. Student results ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...from the drop-down and add an explanation in the adjacent Patent/License Explanation field. Filling ...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...developed to replace helper viruses in AAV production systems. We’re just skimming the surface of the natural... -
6 Tips for Grant Writing
TypeBlog Post...co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the...clear that you are staying because it is the best place in the world for your research to rapidly progress... -
Tips for Technical Support Calls
TypeBlog Post...Google it...Or Yahoo! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet ...experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post... specific transcription factor binding sites interspaced with random sequences. Strategy #4 (ProD): ...where lower levels of labeling makes it easier to trace a neuron’s projections. Generating animal models... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria thus...known antibotics. These might be used on hospital surfaces and in hand sanitizers. There are many clever... -
The Materials Science of Optogenetics Experiments
TypeBlog Post... scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that ...brain tissue a short distance so ideal implant placement should be about 0.4-0.5mm above the site of your... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...teamwork, manage day to day operations within their workplace, and pursue leadership opportunities at the local...postdoc). I soon realized that many of the issues faced by postdocs and institutions are not local but are... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...conferences, but after receiving her degree she was faced with a similar conundrum – she had the skills, training...color changing flower project was inspired by a Facebook comment. In our day job, we worked on plants ... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post..., but those outside of your field can point out places where you gloss over essential details or omit ...avoid rushing out the door without your poster, place it by your keys a day in advance. Dress for the ... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...comfortable with awkward silences but I’ve learned to embrace them after one ended with the person in front of...your manager for advice if you’re not sure how to face a particular situation. But keep in mind that a ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...applications across a wide range of topics. He embraces open science, self-publishing directly on his .... R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore... -
PhD Applications After COVID
TypeBlog Post...simply as it seems laid out above, there are many places where the PhD application cycle becomes much more...program will allow for you to have the time and space to delve into what matters to you. It can also be... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...England Biolabs. Golden gate assembly limitations Embraced by the synthetic biology community, Golden Gate...efficiency, stability, and buffer compatibility have placed practical limits on single- or two-step assemblies... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...so many technologies currently available, you now face the challenge of trying to figure out what system...Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn...