We narrowed to 700 results for: BASE

...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

...genome and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase ...tools such as Cas9-recombineering, CRISPRa/i, and base editing. Leveraging a unique marriage between distinct...

...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...

...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...

...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...

...plasmids she can use as positive controls in PCR-based cervical cancer screens. This third grant represents...

...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...

...References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for...

...vectors are also likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid...

...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

...Bacterial Strains The genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids...commonly used choice. Other options, including four-base (quadruplet) codons, have also been explored. A ...

... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...

...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...

...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and...

... the expertise he’s developed as well as CRISPR-based gene repression tools to find and test genes that...

...team creates stabs for each plasmid being shipped based on the information in our lab software, labeling...

...factor,” a measure that dates back to 1975 and is based on the average number of citations for recent articles...