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We narrowed to 700 results for: BASE

Showing: 401 - 420 of 700 results
  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...
  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...
  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...
  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...
  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...
  6. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...
  7. Editor's Choice, July 2016

    Type
    Blog Post
    ...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...
  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

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    Blog Post
    ...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...
  9. Genetic Code Expansion

    Type
    Collection
    ...Bacterial Strains The genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids...commonly used choice. Other options, including four-base (quadruplet) codons, have also been explored. A ...
  10. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...
  11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...
  12. Deisseroth INTRSECT Collection

    Type
    Collection
    ...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and...
Showing: 401 - 420 of 700 results