We narrowed to 564 results for: BLI

1. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   ...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...

2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   ...option is to perform a hierarchical assembly, assembling fragments in multiple steps to avoid using multiple...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

   ...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...

4. A Deep Dive into BioBuilder

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   Blog Post

   .... (and Mexico in 2018). BioBuilder textbook, published by O’Reilly Media. BioBuilder's open-access curriculum...

5. Plasmids 101: Screens vs. Selections

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   Blog Post

   ...selected growth conditions. For screens, there is no obligatory growth advantage – that is, you are not specifically...

6. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...expression under low temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic...

7. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

9. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

10. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...

11. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

12. RNA Extraction Without A Kit

    Type

    Blog Post

    ... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...

13. Summer SciComm Series: Cognitive Load

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    Blog Post

    ...class. Even so, it’s quite easy (and common!) to be blind to the cognitive load you’re placing on your audience...

14. Antibodies 101: Affinity Reagents

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    Blog Post

    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

15. What's New in CRISPR - November 2021

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    Blog Post

    ... question. Three different labs have recently published work that provides a solution to this problem....

16. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ... can be deactivated using yellow light (590nm). BLINK2 potassium channel - An optimized blue-light induced...

17. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    ...fused the red fluorescent protein (RFP) to a self-assembling protein (SAP) such as a bacteriophage shell protein...

18. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    ...could participate. Despite the group not having a public website, all the slides, handouts, and resources...

19. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ... for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...

20. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed PMID: 26427452. Issa, ...