We narrowed to 451 results for: CRISPR
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TypeBlog Post... RNA amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic...
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Modular Cloning Guide
TypeGuide...multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning (FTK) Fungal Expression, CRISPR Arnold...experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian Expression Takashi...MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering, CRISPR Sylvestre Marillonnet...SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome Engineering, CRISPR, Plant Expression Vladimir...Engineering, CRISPR, Plant Expression Johannes Stuttmann Plasmids for assembly of constructs for CRISPR genome...Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome...(a.k.a. K. phaffii ). NT-CRISPR Plasmid Collection Genome Engineering, CRISPR, Bacterial Expression Anke... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions... -
Common Injection Routes in Mice
TypeBlog Post...resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids ... -
Hot Plasmids February 2024
TypeBlog Post...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...Perkins presented a poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...others at the Sainsbury are working on a lot of CRISPR tools for editing plant genomes. We will definitely... -
Guide to Using Pooled Libraries
TypeGuide...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify... -
Addgene's eBook Collection
TypeGuide...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ... -
Science Guides
TypeGuide...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies... -
Tips for arabidopsis transformation
TypeBlog Post...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13... -
The Challenges of Cell Culture
TypeBlog Post...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over... -
Plasmids 101: Control Plasmids
TypeBlog Post...or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...Representative Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector ...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance... -
Luciferase Plasmid Collection
TypeCollection...enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to...