Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 825 results for: EED

Showing: 401 - 420 of 825 results

  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... feasible, especially when the lentiviral stock needs to be titrated. If storing for less than a day, ...properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service! References...cellular receptors for vesicular stomatitis virus.” Proceedings of the National Academy of Sciences 110.18 (2013...

  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...controls! For every experiment, an investigator needs a standard against which the results can be compared...your experiment includes determining what factors need to be controlled for in order to eliminate any alternative... be plasmid-based depending on the experimental needs. Using our experiment as an example, the expected...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... type of RNA that needs to be made: if you want mRNA (for gene expression) you need to use an RNAP II ...promoter based on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited ...

  4. Five Popular Model Organisms

    Type
    Blog Post
    ...knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing specific plasmids ...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...confirmed that avidin was indeed the culprit in egg white injury by feeding rats purified avidin (Kresege...external sources. Humans absorb most of the biotin they need in the small intestine from the microflora that ...

  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...probably not safe to assume that they saw what they needed to see and just didn’t have anything to contribute... sub-skill to have here is pretty intuitive: You need to be willing to face conflict head-on. That doesn...most frequently towards these approaches, you may need to put more conscious effort into your conflict ...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...backbone: the DNA vector that contains everything needed to replicate inside a host cell but with space ....  We built these plasmids for flexibility and speed, so you can design, test, and innovate faster than... Perhaps these antibodies are just the tools you need to push the field forward! Browse the IPI Glypican...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...plasmid compared to DH5α. However, more research is needed to determine which recA- strains are most effective...2009), pUC multimerization was reduced by growing seed stocks at 30°C compared to 42°C. Screen multiple...production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church lab uses this system...Response Element (TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is...linked in groups of 6), allowing transcription to proceed. When cumate is added, it binds cTA, resulting ...

  10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative...end-joining activity, and for others, there is a need for the exclusive ligation of fully base-paired ...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA sequence encodes the proteins the transposon needs for transposition. Transposition protein: This is... transposons” since they encode the protein they need to move around, either a transposase or a reverse...transcriptase. Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase...

  12. Important Considerations When Using AAVs

    Type
    Blog Post
    ...packaging capacity, AAV vectors were suitable for our needs for the following reasons: Safety: Since AAVs ...should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are relatively quick...useful for a wide variety of experiments, care still needs to be taken when selecting which particular AAVs...

  13. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more... injury in the acutely inflamed glomerulus." Proceedings of the National Academy of Sciences (2016): 201606253...cell subsets in the skin-draining lymph node." Proceedings of the National Academy of Sciences 113.4 (2016...

  14. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the end, this results in an experiment that could need more than 8x107 successfully targeted cells. Depending...the analysis becomes easier, the number of cells needed is lower, and perhaps most important, targeting...good idea to keep a bioinformatician close if you need help putting your list together. Once you have your...

  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...should also be considered. If the light intensity needed for activation is too high, the photoactivation...photoswitching between two fluorescence wavelengths. Indeed, within the world of super resolution microscopy...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

  16. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...grow haywire, access and create more vasculature to feed themselves, use the blood stream to commute to and...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted... inhibit expression of co-stimulatory molecules needed for T cell effector function. Cancer cells also...

  17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...as excitation wavelengths and emission profiles, need to be matched with the experimental setup and objectives...Resonance Energy Transfer (FRET) applications, the dyes need to have compatible excitation and emission profiles...witnessed remarkable advancements, driven by the need for more precise, stable, and versatile imaging ...

  18. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...we’ve included a glossary at the end if you ever need to double-check your vocabulary! A brief review ...double-strand breaks. The only things base editors really need from Cas9 are sequence specificity and the R-loop...efficient and precise editors. Most researchers agreed on the first part of the likely editing mechanism...

  20. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...should our new blog be any different? If you still need an extra push, there’s evidence that laughter really...’s a way to connect with other people. (We don't need evidence for that one, do we?) OK, so, now that ...
Showing: 401 - 420 of 825 results