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We narrowed to 829 results for: EED

Showing: 401 - 420 of 829 results

  1. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their...genuine interest to other researchers in their field. Indeed, sharing results with the broader scientific community...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...of a donor DNA molecule. Your donor molecule will need to have homology to the targeted locus on either...your donor molecule is used as the template, you need to incorporate homologous sequence to the right ...larger knock-in (200 bp – 2 kb), a dsDNA donor will need to be used due to synthesis limits of oligonucleotides...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ...have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers... can still reduce the number of spin columns you need to complete your research. Do you know of other ...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    ...likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for which the development...resistance will be slow. CRISPR may be just the method needed to jumpstart the fight against antibiotic resistance...Thus, a very large number of phagemids would be needed for an in vivo therapy. Another strategy for CRISPR...

  5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...young researchers need to publish good publications in a short period of time and may need to do some fashionable...mechanisms of regulation of genome functions, we need to identify molecules associated with specific genomic...

  6. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...going alone - if you are aware of a problem or a need for something, usually someone else has realized...these types of events can be very tough. First I needed to find the right contacts at the companies and...

  7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter, the Harris...eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA editing efficiency...) to purify single base edited cells without the need for single cell isolation and downstream sequencing...

  8. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...lab and offering advice on areas where you might need some help. Today, we’re highlighting an oft-underlooked...if it were a meeting. Gather the resources you’ll need ahead of time and mute your notifications during...At some point or another, you’ll find yourself needing to brainstorm with a team. One major challenge ...

  9. Lab to Office Culture Shock

    Type
    Blog Post
    ...very likely be on a team that supports you when you need it. The opportunity to be creative and think critically...actually spending doing work. Yes, you may legitimately need to be in the lab that long because of the nature...during the day was also a scientist. But now you also need to talk to Tony from legal, Diana in finance, and...

  10. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...still take a community to advance our knowledge. We need to continue to develop improved, reliable tools ...Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. D., Bishaw, Y. M., Bendrick, J. L... A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V....

  11. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...developing the MoClo system, researchers wisely agreed on universally using the same four-base overhangs... four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate... high number of assemblies, you will also likely need to miniprep a lot of plasmids from your bacterial...

  12. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Just as researchers need to access and reference external data sources, they also need to continue to make... at the characteristics and capabilities an ELN needs to facilitate true reproducibility. The following...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...for a bacterial infection. While this post will, indeed, focus on bacteria, it is important to note that...concentration, or sometimes more exposure time is needed for the drug to work… if the instructions for treatment...or not, because scientists in the laboratory will need to isolate and grow whatever bacteria are causing...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    ...line maintenance The key is good recordkeeping, you need to have a way of tracking how many passages the ...reliable. When you order a new cell line you will need to grow this initial stock and create a set of vials...The future Attitudes are changing but yet more is needed to convince people of some of the basic tenets ...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... feasible, especially when the lentiviral stock needs to be titrated. If storing for less than a day, ...properly and be consistent when following protocols. Need Virus? Check out Addgene's New Viral Service! References...cellular receptors for vesicular stomatitis virus.” Proceedings of the National Academy of Sciences 110.18 (2013...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...controls! For every experiment, an investigator needs a standard against which the results can be compared...your experiment includes determining what factors need to be controlled for in order to eliminate any alternative... be plasmid-based depending on the experimental needs. Using our experiment as an example, the expected...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... type of RNA that needs to be made: if you want mRNA (for gene expression) you need to use an RNAP II ...promoter based on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited ...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...probably not safe to assume that they saw what they needed to see and just didn’t have anything to contribute... sub-skill to have here is pretty intuitive: You need to be willing to face conflict head-on. That doesn...most frequently towards these approaches, you may need to put more conscious effort into your conflict ...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ...knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing specific plasmids ...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...confirmed that avidin was indeed the culprit in egg white injury by feeding rats purified avidin (Kresege...external sources. Humans absorb most of the biotin they need in the small intestine from the microflora that ...
Showing: 401 - 420 of 829 results