Site Directed Mutagenesis by PCR
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Blog Post
...reduction: a method for analyzing the role of renaturation temperature in the polymerase chain reaction...
We narrowed to 447 results for: ENA;
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Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...discovery of a phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation... -
Evolution of Lab Techniques
TypeBlog Post...Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia." New England Journal... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...which, by undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... the repair of breaks that lack a second end to enable DSBR or SDSA. Homology directed repair and genome... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...module that can report on location. This approach enables the study of proteins in cells, but not other biomolecules... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I thought... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...intensity of the fluorescent signal is boosted and enables single molecule tracking in living cells. The HA... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...compared to wild type BirA. TurboID and miniTurbo both enable biotin labeling in 10 minutes without any issues... -
Five Popular Model Organisms, Part 2
TypeBlog Post.... (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...PubMed Central PMCID: PMC5811451. Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore: a bright... -
Plasmids 101: Biotinylation
TypeBlog Post...determined to be due to a deficiency in Vitamin H later renamed Vitamin B7/biotin. Egg white injury would plague... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...conversations like those at the FOR symposium and will enact their solutions as soon as they become actionable... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Wu, Y., Ye, C., Lin, K., & Xu, F. (2023). AAV11 enables efficient retrograde targeting of projection neurons... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...technology, however, is that the transposase also enables the excision of the transposon in a completely ... -
Finding Your Perfect Job After University
TypeBlog Post...and how I felt about the different positions to enable them to decide for themselves. Here is my experience... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...Levene SD. Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis. Methods Mol Biol... -
Plasmids 101: Repressible Promoters
TypeBlog Post...ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1 activity... -
CRISPR 101: Off-Target Effects
TypeBlog Post...nothing to worry about all. Let’s consider several scenarios below for a CRISPR experiment which involves making...