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We narrowed to 520 results for: HA tag

Showing: 401 - 420 of 520 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...the post) has a number of helpful suggestions.  Gels Gel issues usually come down to voltage and time....ladder. Unstained ladders contain either ‌a protein tag or an IgG binding site, allowing you to visualize...bands, your voltage is likely too high. If you’re seeing a smiley face at an appropriate voltage (usually...can happen with large proteins, try lowering the voltage and using a longer run time.   Samples can run ...packs can help keep the system cool, as can lower voltages.  If your small protein is over-transferring, ...transfer Increase transfer time Increase voltage Increase SDS/decrease alcohol in transfer buffer...Psi membrane Semi-dry transfer Decrease voltage Decrease transfer time Decrease SDS/increase...

  2. Summer Fun at Addgene!

    Type
    Blog Post
    ... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...Social Media Are you following us on Twitter or Instagram (or Facebook or LinkedIn) but want to hear and...love sunshine and warm weather - check out our Instagram all summer long to see what kind of shenanigans...

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

  4. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its developers...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ...that particular organism available at Addgene. Percentages represent the fraction of that total with the...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study ...guide sgRNA-Sirius-8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...supernatant during the production phase. However, this has not been well characterized as it is not a common...microvesicles as a vehicle for drug delivery that has recently gained interest special contexts, such as...researchers discovered that these particles have some advantages over the conventionally purified AAV vectors....

  7. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient...together, there are occasional errors. That can be advantageous if you are trying to knock out a gene. One can...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Cytotoxicity can also be cell type dependent: if a cell has few receptors for a specific envelope protein, it...lentiviral vector to be transported retrograde and also has higher neuronal transduction efficiency and a better...different viral membranes, tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table...its own, it needs to be pseudotyped. This takes advantage of the EnvA-TVA receptor-envelope system (Wickersham...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial and yeast cells...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...DNA without a kit. DNA purification kits have advantages: they are convenient and provide uniform, consistent...least entraps nucleic acids. The Botella lab took advantage of this property and developed a protocol that...

  12. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...this past year and a half. My wife and I went to Patagonia in February of 2020 and came back just a week ...

  13. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...showcase it all!           View this post on Instagram                       A post shared...dyed with real microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab...

  14. Science Communication for Everyone

    Type
    Blog Post
    ...cornerstone for The Addictive Brain. Our community has also requested live question and answer sessions ...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...background. Social media is a powerful tool. Use hashtags effectively. This would help you direct your content...

  15. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities...contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable.... A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate ...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses...acid-based vaccines. These vectors provide several advantages over traditional vaccines, including 1) cellular...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...many academic scientists are starting to see the advantages of using social networking to start, build, and...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...   In conclusion, the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal...editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular genomic editing systems...the newly generated pEcCas/pEcgRNA system.   Advantages of the pEcCas/pEcgRNA system Compared with the...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) 1X PBS pH 7.4 without calcium...Primers/probe targeting RRE: forward primer: tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer...inactivated premium grade, fetal bovine serum 5 mL glutaGRO 50 U/mL benzonase: 15 mL DMEM Complete 3 µL of...
Showing: 401 - 420 of 520 results