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We narrowed to 982 results for: IGH@

Showing: 401 - 420 of 982 results

  1. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...their regenerative abilities is expected to shed light on human aging pathological developments (Vieira...amphibians in science with early research providing insight into some of the most significant and fundamental... Image from Holger Krisp. The frog genome has high synteny with that of humans so they are often used...the world. With its extensive scientific history, highly characterized genome, and widespread utilization...produces many seeds), self fertilizing, a small yet highly characterized genome, and is easily maintained ...Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature 428, 493–521 ...

  2. Editor's Choice, July 2016

    Type
    Blog Post
    ... To better highlight the great content contributed by our bloggers each and every month, we've decided...Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the excitement surrounding anything...month is from guest blogger James D. Fessenden at Brigham and Women's Hospital. This post describes how to... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Every few months we highlight some of the new plasmids, antibodies, and viral preps in the repository...deactivation). Both have significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited...and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones...Protein Innovation (IPI) recently released a set of highly-specific recombinant antibodies against three of... — with efficiencies between 5% and 11%. It has higher efficiency and lower indel formation than methods...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...not having any confounding mutations, the risk is high. You are generating a gene therapy product with ...clinical trials. An off-target rate of 5% is far too high when considering human patients and the diseases...measurements at those sites to get an idea of how high the off-target frequency is (more on this below)...sequencing methods. Candidate site sequencing Sites with high sequence similarity to the gRNA can be sequenced...a DNA mutation, off-targets must be quantified slightly differently. For a comprehensive approach, RNA-seq...library screens, the same principle holds true - the higher the number of distinct editing events, the lower...

  5. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...fixed sample. Depending on the sample type, this might be frozen or formalin-fixed, paraffin-embedded tissue...Depending on your needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B).... Depending on the goal of your experiment, you might need to quantify the intensity of the different ...spatial relationship between the targets. Or, it might be sufficient to describe or classify the labeled...label a sample that expresses your target(s) at a high level, either naturally or by overexpression, for...similar between the two experiments. If not, it might be that the other antibodies are disrupting the ...

  6. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...fortunate that there’s a guide that directs cleavage right before the stop codon!  Figure 3: How to ...guide sequences (arrows) and their PAM (bold highlighted text) and cleavage sites (slashes) are shown....plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_C1! After creating...primers to add T7 promoter-left homology arm and right homology arm to the desired insert (e.g., mScarlet...the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template.   ...

  7. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...RNA is highly desirable for extending the complexity of genetic circuits, allowing for tighter spatio-...Illinois at Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive..., I think, are endless.” Abil says the new tool might even find therapeutic application in addition to...she recommends using standard BsaI in place of the high fidelity version (despite what the original paper...

  8. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...vectors containing high numbers of gRNAs in one overnight reaction. STAgR is highly customizable as vector...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....

  9. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ...connection to the hiring manager. They might say yes and they might say no but do not confuse networking...scientist in the academic system has many pluses. I delighted in my graduate school years for allowing me to...personal questions can elicit the most valuable insights you will get from the interaction. Most people...great way to practice for real interviews where you might spend a whole day having to talk with strangers....

  10. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you might have...plenty of delicious desserts. We enjoyed a video highlighting the changes at Addgene over the last 10 years...Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents up ...

  11. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...customized to highlight different skills and to get the important information in the right place on the...organization is small, any person at the company might be able to help you. If the organization is large...additioanal advice for the science career search.  You might also enjoy the other posts in the Addgene Blog Scientist...

  12. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...and can exhibit recombination levels as high as 1-3%. In light of this finding, we now include guidance...leaky expression. On the right non-homologous scrambled recombination sights lead to reduced reversion...strategy should work, these vectors suffered from high levels of spurious expression.  As such, researchers...weak and may require longer waiting periods and/or higher injection titers to achieve adequate expression...

  13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... homozygous knock-in animals can be obtained in eight days. The only animals on a plate, other than the... delivery, screening, and more. Here are some highlights from the past few years:  Repair template design...improvements from the Mello lab resulted in strikingly high editing efficiencies, obtaining as many as 100 independent...corresponding to the same strand as the PAM produced a higher editing efficiency, while for edits 3’ to the PAM...templates. They found that Cas9 RNPs could be toxic at high concentrations, and editing efficiency improved ...

  14. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...transposons evolve under contrary selection pressures: high efficiency helps them spread, but also stresses ... evoCAST In the emerging world of gene therapy, highly tailored treatments to specific mutations require...long sequences. In their experiments, PASSIGE had higher efficiency, but evoCAST required less optimization...optimization for new targets and produced a higher-purity product, likely because the integration occurs in a... can’t wait to see where evoCAST takes us on the high seas of gene editing. Bon voyage!   References...

  15. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...proximity to an endogenous gene. Each trap uses a slightly different approach (described in detail below)...for the endogenous gene – its expression levels (high/low) and patterns (tissue/timing) can be monitored...screened. Depending on the marker, traps can be used as high-throughput screens to introduce reporters throughout...the top trap – they don’t require insertion in a highly specific gene region and they don’t disrupt the...Resources References Wood, V., et al., Hidden in plain sight: what remains to be discovered in the eukaryotic...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...are very valuable biological tools, and with the right safety considerations, they aren't dangerous for...transductions can offer benefits such as tissue-specificity, high efficiency, and can be selected to be either integrating...our viral selections to see if we have one that might work for your next experiment. Using transduction...Answering these questions will help guide you to the right method for your experiment.  A cool feature of viral...virus can sound daunting, but it is easier than you might think. We have protocols available to get you started...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... TX-TL system is capable of robust expression of high G+C content genes, as validated through monitoring...CRISPR Plasmids and Resources page. Here are some highlights from the past couple months including several...

  18. What the HEK?

    Type
    Blog Post
    ...an advantage to use the cell line and we will highlight some of those applications here.   Virus production...transfect in bulk and produce recombinant proteins at high levels. In a case where clinical applications of...and if your research doesn’t allow for that, HEK might be the cell line for you. Are HEKs for you?   If...you are looking for a human cell line model, HEKs might be a great option! Especially if your research interests...

  19. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...yet familiar. The idea that my piecemeal degree might one day foster a meaningful day job only came into...enjoyed a taste of what was to offer, continued straight on to a master's degree in science communication... one day required of me for work, I would have a high level of career satisfaction indeed.   For many ...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all...law to poetry. A research discipline in its own right, science communication reflects a necessary confluence...

  20. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...In a big lab, the advisor might not be around much and your real boss might be a postdoc – this isn't ...competitive style to spur you to succeed or you might enjoy working closely with others to accomplish ...before you take the position. Am I choosing the right mentor? Make sure your advisors will be good role...candidate to ask lab heads, lab members and lab neighbors during the interview process. What if I want...
Showing: 401 - 420 of 982 results