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We narrowed to 1,034 results for: LAS

Showing: 401 - 420 of 1034 results

  1. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...sequencing on select regions of all distributed plasmids as part of quality control, but does not perform...unlike an RNA guide, it cannot be produced from a plasmid in cells. Additionally, in vitro assembly of NgAgo...with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself ...complexes or with guide RNA- and Cas9-expression plasmids. Cas9 guide RNA delivery does however rely on ...(1), NgAgo has received much attention and the plasmid has been requested by labs around the globe nearly...happy genome editing! Check Out Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest ...

  2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...generate stable cell lines. Packaging and Envelope plasmids provide the necessary components to make lentiviral...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ... tag expressed on a plasmid, allowing for in vivo biotinylation through a plasmid-based biotin ligase....CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...which can easily be cloned into a gRNA expression plasmid. Experimental use Since the initial report describing...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow... where variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...(cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library by inserting...Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to...region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence...sequence. When viruses produced from this plasmid pool are injected into an animal with cell-type-specific Cre... do these AAVs transduce the CNS and PNS? Both plasmids infect the PNS or the CNS much better than the...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...fluorescent conjugates into cells. Fortunately, over the last 20 years clever chemists and biochemists have developed...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...off a flurry of activity and the majority of the classic small molecule fluorophores, such as rhodamines...small-molecule labeling techniques have focused on this classic, net-neutral dye scaffold due to its established...enhance the brightness and photostability of the classic rhodamines and their analogs without sacrificing... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

  7. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...system as a robust and popular lab tool. In the classic two hybrid system (also called the interaction ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...positive control to ensure that your setup works. The last thing you want is to run your lovely library screen...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  8. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

  9. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile...upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the...detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound...difficult-to-study RNA-modifying enzymes, such as the RNA demethylases FTO and ALKBH5. Their ease of production have...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain multiple ...the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used this information...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

  15. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...

  16. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...

  17. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

  18. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... Surface labeling is particularly useful for classifying and sorting cells by identifying lineage markers...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live cells... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality for...infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk for other chronic...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

  20. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine base editors (CBEs...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...
Showing: 401 - 420 of 1034 results