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We narrowed to 1,038 results for: LAS

Showing: 401 - 420 of 1038 results

  1. Tips for Technical Support Calls

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    Blog Post
    ..., make sure to mention the plasmid catalog number, or at least the plasmid name in your initial query....from researchers, and the vast majority of our plasmids and pooled libraries were originally described...links to later papers that successfully used said plasmids. We provide these references as our way of saying...to keep in mind after you hit “send” or dial that last digit: An answer should come soon, but not instantly...Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help with the Addgene...

  2. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...field of synthetic biology. The first was a new class of programmable riboregulators, called toehold switches...detect the virus directly from infected monkey blood plasma without purification or concentration of the virus...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  3. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post
    ...imperative to the health of an organism that both classes of genes function normally to maintain balance ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

  4. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ...sequencing on select regions of all distributed plasmids as part of quality control, but does not perform...unlike an RNA guide, it cannot be produced from a plasmid in cells. Additionally, in vitro assembly of NgAgo...with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself ...complexes or with guide RNA- and Cas9-expression plasmids. Cas9 guide RNA delivery does however rely on ...(1), NgAgo has received much attention and the plasmid has been requested by labs around the globe nearly...happy genome editing! Check Out Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest ...

  5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post
    ...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...generate stable cell lines. Packaging and Envelope plasmids provide the necessary components to make lentiviral...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide...

  6. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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    Blog Post
    ...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, 2021...re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries, kits... 2021) 1.5 million plasmids shared in total (currently at 1,484,507 as of April 8, 2021) 5,000 depositing...addgene.org homepage! You can track the number of plasmids right below the search bar and more stats further...

  7. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow... where variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  8. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...(cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library by inserting...Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to...region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence...sequence. When viruses produced from this plasmid pool are injected into an animal with cell-type-specific Cre... do these AAVs transduce the CNS and PNS? Both plasmids infect the PNS or the CNS much better than the...

  9. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post
    ... tag expressed on a plasmid, allowing for in vivo biotinylation through a plasmid-based biotin ligase....CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...which can easily be cloned into a gRNA expression plasmid. Experimental use Since the initial report describing...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated...

  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ...fluorescent conjugates into cells. Fortunately, over the last 20 years clever chemists and biochemists have developed...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...off a flurry of activity and the majority of the classic small molecule fluorophores, such as rhodamines...small-molecule labeling techniques have focused on this classic, net-neutral dye scaffold due to its established...enhance the brightness and photostability of the classic rhodamines and their analogs without sacrificing... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

  11. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...system as a robust and popular lab tool. In the classic two hybrid system (also called the interaction ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...positive control to ensure that your setup works. The last thing you want is to run your lovely library screen...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ... Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...recent years. TnpB nucleases represent an exciting class of compact genome editors, typically only around...for ωRNA (reRNA) expression (see Figure 1). Both plasmids contain a hygromycin resistance gene as the plant-selectable... 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by Sanger...primer. Step 7: Transformation Your confirmed plasmid is now ready for Agrobacterium-mediated, biolistic...

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the...detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound...difficult-to-study RNA-modifying enzymes, such as the RNA demethylases FTO and ALKBH5. Their ease of production have...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

  15. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

  16. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

  17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile...upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

  19. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain multiple ...the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used this information...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...

  20. Addgene’s New Way to Help You Share Science

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    Blog Post
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...
Showing: 401 - 420 of 1038 results