Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 808 results for: MAK

Showing: 401 - 420 of 808 results

  1. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...that commercially available cloning kits can often make the process much more streamlined, because they ...the reaction in favor of the desired assembly to make the process close to 100% efficient. Gateway cloning...Multiple inserts: There are some cloning methods that make working with multiple inserts a breeze. Among them...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ... any features of the library backbone that would make the library more difficult to work with. For example...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ... reference protocols There are many decisions to make when developing your amplification protocol! We ...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...modulators regulate transcription and chimeric efforts make a precise edit or insertion via a chemical modifier...but it certainly suggests that Addgene depositors make CRISPR tools that fill needs in the community. The...centers around the impact of science, but our data makes it clear that it’s also a story of community, cooperation...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...School of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more...Parents of infants will have no problem with this!) Make sure your colonies are well separated. For this,...taking both early- and late-arising clones, and making sure we always maintain a backup replicates of ...

  5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...system), and the third post will describe how you can make your own mutant flies. Find Drosophila Resources...bristly hairs. Females do not have sex combs. To make genetic crosses properly, it is important to isolate...emerging at night. An incubator with a light cycle makes for very predictable work! When flies first emerge...

  6. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...the localization of your protein of interest is to make it detectable. Visualization can be achieved using... and their potential overlap, it is essential to make sure that the selected FPs do not influence each...your next localization experiment and invite you to make any fluorescent protein constructs you create in...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...about using non-homologous end-joining (NHEJ) to make deletions or homology-directed repair (HDR) to create...very small homology regions (5-25 bp) for repair, making it easier to construct targeting vectors. Addgene...downregulated, MMEJ represents another method for making targeted modifications. A recent publication by...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...Maity et al., 2015). If your antibody is not IgG, make sure to look up the setting for your isotype.  Antibody...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...researchers need to reflect upon before they attempt to make essentialist connections between biology and ancestral...Map (DepMap), a cancer dependency database used to make this information accessible to the research community... at all. This unexpected discovery, which didn’t make sense in any kind of biological framework, suggested...

  11. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...below). Addgene scientists inspect each sample and make sure they have been barcoded with a unique ID number...therefore particular sample is missing so that we can make a new tube from a replicate sample. The transformation...replicate and retain the plasmid. This allows us to make unlimited copies of each plasmid so that we never...

  12. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...You will often hear the term ‘grant writer’, but make no mistake, researching, winning, and effectively...writing. In fact, many grant applications and grant makers have moved away from long written proposal narratives...relationships with foundations and government grantmakers. We keep up-to-date on the changing guidelines...

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...also helped us find areas for improvement. We will make a greater effort in the future to match people within...Binning Singletons is its own organization, working to make scientific meetings more productive for attendees...

  14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zebrafish embryo develops outside of the mother, making them ideal for studying vertebrate embryogenesis...zebrafish To highlight some of the features that make zebrafish useful, I’ll outline two recent lineage...unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow” fish lines have been...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    ...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene...come up with a list of things to consider as you make this transition. Of course, a “desk job” could entail...

  16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... Here you will find some useful tips to help you make your plasmid construction with MoClo a walk in the...units. Created with BioRender.com.   Planning makes perfect Our first tip, and arguably the most important...cloning success depends directly on the choices you make during the early stages of the process. Some questions...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import... to increase accessibility to our repository by making it easier to find materials and learn about them... know we will continue to ask “What can we do to make our materials both available and accessible to scientists...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...truncated forms that limit its functionality. To make biophysical and structural studies more feasible...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... bench. Specifically, roles in technical support make excellent use of the skills developed by life science...advantageous to speak multiple languages - that makes communication with customers from around the world...convenient alternative for asking a quick question or making initial contact, but is less suitable for troubleshooting...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    ...well-researched field, you may need to get specific to make sure your article doesn’t turn into a textbook. ... short bursts (even twenty minutes was enough to make some progress). For most researchers, it’s not always... into something cohesive-- change the sentences, make it flow, and start telling the story. Like any editing...
Showing: 401 - 420 of 808 results