We narrowed to 525 results for: MEA

1. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   ...dangerous, they also provide researchers with new means to deliver DNA and other molecules to host organisms...

2. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...cleaves outside of its recognition sequence. This means that each component can be designed with an overhang...

3. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...remove some of your sample —and harsher stripping means more sample loss. If you are still optimizing your...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ...independently of dCas9 targeting (Galonska 2018). This means that off-target loci – in neighboring chromatin ...

5. Hot Plasmids - October 2020

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   Blog Post

   ...proliferation and had a greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed...

6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   ...small size of Acr proteins (~50-200 amino acids) means they can be delivered in vivo using AAV vectors....

7. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ...enzyme recognizes a PAM sequence in your site doesn’t mean it’s suitable for your experiment. If you’re looking...

8. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...selective effector molecules (PSEMs). Higher potency means lower drug doses, which decreases the risk of off...

9. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   ... poster should be legible from a distance. This means big font and fewer words. Further, the poster should...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...faster in the following test and skew results measuring locomotion. Mice may need to be given some time...

11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    ...alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene...

12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    ..., Rinaldi)! But leaving a PhD program doesn’t mean you’ve failed. “I got a job as a research assistant...

13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six...

14. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic showing...

15. Engaging with science and society at pgEd

    Type

    Blog Post

    ... of genetics and our consistent stream of events mean that there is never a dull day in the office. And...

16. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ..., at Addgene we typically concentrate 100-fold meaning that a 10 cm2 dish preparation of virus will only...

17. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...that give the strongest biotinylation signal, as measured via Western blot, and that result in proper localization...

18. Lab Automation at Addgene

    Type

    Blog Post

    ...about more lab updates as we develop them. In the meantime, if you have any great lab automation tips and...

19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...involve identifying a therapeutic gene, finding a means to deliver it and identifying a suitable route of...

20. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ... for delivery to the cells. As a precautionary measure, it’s also good to make a few single base pair ...