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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 818 results for: Mal

Showing: 401 - 420 of 818 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version...neuron-specific expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors...neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible with constitutive...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... to probe cells and circuits without disrupting normal brain function. Chemogenetics has become a popular...successfully reach target cells while resulting in minimal off target effects. For example, if your receptors...endogenous gene expression, which can lead to changes in normal cell physiology and function (Saloman, JL, et al...

  3. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...western blot Run your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to...first. Then continue on with your western blot as normal! Stripping your membrane Alright, so you’ve dried...antibody, you’re ready to repeat your western blot as normal. Be sure to rinse away the secondary antibody you...

  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...on each and every isolated plasmid sample and normalize each to fall within a 10 fold range. Plasmids ...Since NGS assembles a larger sequence from many smaller sequences, regions with repeats may not assemble...samples are filled into the same process as our normal Complete Plasmid Sequencing (CPS) workflow and ...

  5. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Communication Skills 5. Practice makes perfect, apply for small grants too There are actually quite a lot of different...available for researchers, so look into applying for smaller grants from within your institute or a private ...certainly found that the experience I gained in writing small travel grants to attend conferences and a seed funding...

  6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...this: Rank = (compressed CFD score) * (gaussian normalized cut-to-insert distance) This gives each guide...insert, the more poorly the guide performs HDR. Optimal mutation in-depth One the most sophisticated features...mind, we implemented the following algorithm for optimal mutation in CrispyCrunch: If the guide PAM resides...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...buffer exchanged virus, remember to make one or two small aliquots to use for qPCR titering. This helps avoid...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch...makes AAV a versatile tool for gene expression in mammals Use the PEI calculator for planning AAV packaging...

  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At...stayed until he graduated. Years later I attended a formal training for new managers and I had a “Eureka” ...was not engaged because I only left him/her with small tasks and s/he was always asking for more work (...

  9. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface...student-centered SMART Team modeling program in which small teams of students work closely with local researchers...Center for BioMolecular Modeling, we spun off a small, family-run business – 3D Molecular Designs – to...

  10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...serotype of the prep by interrogating the VGS data for small predetermined seed sequences unique to the capsid...to transgene expression in recombinase-negative animals, producing misleading results.  To date, we have...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...and utilize both a genetic component as well as a small molecule which we will describe in more detail below...contain a FRET pair, a genetic component, and a small molecule component. Early versions of these sensors...component is delivered in vivo or in vitro and a small molecule is added exogenously to measure a phenotypic...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions... deletion. However, there is the chance of a chromosomal inversion, or just random mutations if the chromosome...

  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...locus when complexed with an appropriately designed small RNA, or guide RNA (gRNA). The gRNA conventionally... need to generate new cell lines or transgenic animals expressing dCas9 as a means of modulating gene ...: 26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering...

  15. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...university with the community. I even spray painted a small erlenmeyer flask with gold glitter spray paint to...Museum and others when I was trying to find local informal science educators, and ever since, the State Museum...the network I built during graduate school is my informal advisory board. Although my goals may not have...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...can be used with 3DISCO to image non-transgenic animals such as rats (17).  In addition, the use of viruses...over labeling methods as compared to transgenic animals (17).  These viral plasmids are available through...relationship of the central nervous system under normal and diseased conditions.  Thank you to our guest...

  17. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...Charter and it has really strengthened the network of female scientists. Twitter has been really good for that.... I’m more in touch with a remarkable number of female scientists whose tweets I find really inspirational...about how much that’s on it are plants, and the animals you eat ate plants too. Think about how many people...

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... or scientific name into the Organism box. This small piece of information can significantly reduce your...Unlike megablast, the regular blastn program uses a smaller word size and lower scoring penalties for mismatches...desired analysis, BLAST may not always be your optimal choice. For difficult sequence alignments that ...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...combination of the three (Servellita et al., 2023). Normally when children have acute viral hepatitis it is...commonly derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family,... adenoviral vectors in Addgene’s repository is smaller than for other vector types, we hope that this ...

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...more focused format, where all students work on malate dehydrogenase (MDH), a citric acid cycle enzyme...characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry...laboratory – a semester-long research approach using malate dehydrogenase as a research model. Biochemistry...
Showing: 401 - 420 of 818 results