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We narrowed to 876 results for: NSI;

Showing: 401 - 420 of 876 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...

  2. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...how Protein A and Protein B interact. You read extensive research on the two proteins and come up with ...antibody against Protein B. The antibodies are both extensively published, knockout validated, and work in your...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...gene expression in a target cell. Practical considerations If you have high quality AAV, you can address...required for the transduced cell to become light responsive than fluorescent proteins required to get a good...interior, causing less particles to be released into suspension and lowering the number of viral particles that...

  6. The MTA at Addgene

    Type
    Blog Post
    ... institutes, the Tech Transfer department is responsible for managing research use agreements like MTAs...beginning makes the sharing process smoother, more consistent, and more pleasant for everyone, which in turn...including MTAs, is perfect, but it does provide consistency and transparency in an otherwise messy process...

  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...caspases are activated last in the cascade and are responsible for the degradation of over 600 cellular components...detected fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu ...developing forebrain and retina. This pattern was consistent with TUNEL staining, a popular method to observe...

  8. Hot Plasmids - January 2023

    Type
    Blog Post
    ...applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright...than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light and electron...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains...

  10. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...space where BosLab exists today. Back then, it consisted of “wooden slab benches” set on top of filing ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming...biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules....

  14. How to Lead a Great Meeting

    Type
    Blog Post
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...meetings tend to fill the shape of their container. Consider whether you actually need a full hour to achieve...FInd Tips on Picking a Research Project Read Other Inside Addgene Posts Resources on the Addgene Website...

  15. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... perhaps surprising, yet important, factors to consider when seeking your dream lab. The principal investigator's...want a non-academic career path?  If you are considering this, make sure to choose an advisor that supports...where the topic of your research can make your transition easier – choose a lab that works on problems ...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... Temperature inducible promoters Temperature sensitive expression systems are typically less leaky than...Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light is absent, YFI phosphorylates...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...this solution wasn’t practical due to the low intensity of luminescent proteins. To make luminescent imaging...luciferase has a low quantum yield, and when transiently expressed in cells, generates much less signal...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

  18. Clear and Accessible Writing

    Type
    Blog Post
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured...did! From Addgene's very beginning, we had to consider digital accessibility for our website and online...

  19. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... mammals, which is thought to be due to their propensity to integrate into proto-oncogenes and disrupt...alternative virus, or other strategies may need to be considered if size constraints are an issue. Adenovirus...Adenovirus is an effective option if you need high, transient expression of a genetic cargo or have a very large...

  20. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...repair template (sister chromatid or homologue) or extensive DNA synthesis. NHEJ also finishes repair of most...streamlined version of this pathway and does not consider the missing or damaged nucleotides that are common...Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References...
Showing: 401 - 420 of 876 results