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We narrowed to 1,020 results for: NTS;

Showing: 401 - 420 of 1020 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...such as adjuncts) and graduate and undergraduate students are exempted from the law. However postdocs, staff... effects on higher ed had been seized upon by opponents of the ruling. However when the ruling was issued...Overtime Rules, made clear that institutional arguments were not as convincing as the recommendations ... therefore the numbers of postdocs at different points on this salary range. We have been able to gather...the institutions that are meeting the salary requirements of the FLSA. In the University of California...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...accept these recommendations, and make gradual adjustments towards this new level. Indeed some institutions...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...important binding events take place during the course of infection. These binding events can be impaired...the most out of your lentiviral transduction experiments. Love your cells and they'll love you back ... problem that often plagues labs and hinders experiments is mycoplasma contamination. Mycoplasma contamination...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...collect and pool the harvests from multiple time points while others opt for a single harvest after transfecting...of infection (MOI) for predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed...Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...some types of research, raw data requires huge amounts of storage. For example, mouse behavioral geneticists...different occasions via video, generating huge amounts of data. When published, this data is usually condensed... of data is essential for efficient science. Improvements in our discipline for annotation and archiving...not finalized its definition of necessary data requirements and likely will not do so (calling it a “sisyphean...another issue of proprietary data formats. Many instruments store raw data in formats that require specific... and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload the burden of data...rely on many other labs to teach us how to do experiments and understand their data. Likewise, we want ...

  4. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...chat for Early Career Researchers.  Many of the participants had certainly realized the importance of seeking...What makes a good Mentor?” Some of the Tweeted comments: “A good mentor has your goals/needs/situation...most of them Is not threatened by the Mentee’s talents or ambitions Encourages the Mentee to take risks...thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab. If only every science trainee...their career. “I want to be a PI that holds my students accountable for their actions and pushes them ...without being taken advantage of, and to respect my students as developing colleagues even as I reinforce the...the necessary power dynamic between advisor and students.” Stay tuned for the answers to the other six ...

  5. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...Their small numbers do not, however, make these patients suffer any less acutely.  The Rare Genomics Institute...those who suffer from rare diseases by connecting patients to important resources and promoting research ...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...accelerate rare disease research and provide hope to patients suffering from these devastating afflictions. ...motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal Mesothelioma  Noushin...mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma. Dr. Nabavi and colleagues... lines and assess their tumorigenesis. These experiments will serve to validate these candidate genes ...

  6. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding...breeding methods have shaped the plants that we grow and eat, and genetic engineering will continue to improve...transient and stable gene expression applications in plants. This smaller, easily customizable vector functions...biolistic methods, making it useful for engineering plants that are not susceptible to Agrobacterium infection...select both transformed Agrobacterium and infected plants. Further opportunities with pSiM24 While these ..., three MCS regions permit multiple regulatory elements to be inserted, further customizing gene expression...as well as stable expression of foreign genes in plants." PLoS one 9.6 (2014): e98988. Pubmed PMID: 24897541...

  7. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...first in a series that will feature advice for students, postdocs, and young Principal Investigators (...alerts for new papers with keywords or a Table of Contents email (eTOC) alert for every new issue of your...careers outside of academia Recently, graduate students and postdocs have become more aware of careers...all the real life skills that employers love PhD students to have: working to deadlines, writing up clear...their personal goals to their career choices.” She points out that a PhD teaches analytical and critical ...outside of academia. She has had previous graduate students and postdocs go on to work in patent law, business... Your Lab,” “Fun in the Lab!" and more.   Acknowledgments Thank you again to Connie Cepko, Tom Ellis...

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous variants have been...These trials unfortunately resulted in several patients developing T-cell acute lymphoblastic leukemia...titering IDLVs include detecting vector particle components or performing quantitative PCR titration of vector...research, we wish you the best of luck with any experiments involving IDLVs. And remember: You can never ...Consequences on Host Gene Expression. Viruses: Essential Agents of Life, 147–175. https://doi.org/10.1007/978-94...leukemogenesis following gene therapy of SCID-X1 patients. The Journal of Clinical Investigation, 118(9)... Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to ...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...DNA-based reagents are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...high binding affinity to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango...useful for both live and fixed cell imaging. New ingredients in the aptamer soup In addition to these established...

  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... and quality for HIV-1 patients. However, ART is not an HIV-1 cure. In patients who stop ART, viral levels... latently infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk ... isolated CD4+ T-cells from two HIV-1 infected patients. Although this is an exciting finding, it’s important...important to note that these HIV-1 patients were ART-naive, so this experiment models the active HIV-1... ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these...CRISPR gRNAs require DNA sequence-specific binding. Patients’ HIV-1 genomes will need to be sequenced to determine...discouraging, it’s important to note that these experiments were done in cell culture, and the cells infected...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... Designs Consulting (EDC), we instead have our clients focus on their experimental needs and consider ...accomplish your science, and can include: equipment, reagents, animals, glassware, and even people. When developing...even includes clocks, timers, and phones.  As for reagents and consumables (such as pipet tips, glassware...to need a tech because you’ll be doing mouse experiments, start defining keywords you’ll want to find ...their goals, rather than focusing on long term investments. For example, purchasing “used” equipment can...resource budget, and know how to decide which components are the most critical to your research, negotiations...limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications...

  12. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...some extra guidance to understand the specific components that make up a plasmid and why each is important...spend more time developing cleverly designed experiments and innovative solutions necessary for advancing...naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids provide one or more functional....   Figure 1: Map of a plasmid with its elements described below.   Vector Element Description...as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later work....plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify and ...Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick contributed to the writing...

  13. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...awareness for rare disease and helps patients find the correct treatments, if available. As part of the team...play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running ...triggers appropriate bone regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic... But, as we in the scientific community know, treatments that work well in animal models do not always... Disease Researchers Check Out Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1...

  14. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...to address the comments to “you” rather than “the authors.” By addressing the comments to “you” I can ... figures). After finishing my section-specific comments, I re-read what I wrote about the paper after ...in the first (and clunky) version of my general comments and adjust them if my opinion changed after this...that there is another human who is reading your comments. Receiving a peer-review is already a humbling...young scientists that are reading your peer-review comments will learn from them and propagate this culture...ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation in their roots...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...oligomerization can lead to misleading artefacts in your experiments. Read this post from Erik Snapp, Associate Professor...oligomerization can be and how to avoid it in your experiments. Better Dyeing Through Chemistry & Small Molecule...fluorophores can still be used in a wide variety of experiments. Read more to find chemical fluorophores for ...and/or engineer “temporally ordered biochemical events” such as those that occur during development. Read...study basic virus biology while leaving virulent components behind. Minigenomes are stripped down viral genomes...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make these libraries...

  16. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...connection between molecular- and cellular-level events and behavior. It’s a problem that he considers ...change has a particular advantage for observing events taking place inside cells that are on the move,...Methods, the FRET changes of these Twitch sensor variants were optimized by testing various linker configurations...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat... than intensity-based readouts.” Griesbeck also points out that the approach he and his colleagues took...“trick” to the development of additional color variants. This resulted in the construction of redCALWY...redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor ...

  17. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...Together, these limitations create challenges for measurements needed to move discoveries from the lab bench...compatible with both intra- and extracellular environments, making it effective for analyzing real-time...biology. They first identified that in a set of patients with gastric cancer (GC), TP53 was one of the ...mutated genes. Next, the authors explored how these variants of p53 shape chemosensitivity and mechanistic ...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the...which was slowed by knockdown of UBE3C or other components of the degradation pathway. HiBiT enabled the...through extracellular-specific and full lytic measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time...

  18. The MTA at Addgene

    Type
    Blog Post
    ...that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized tracking...and developments.  MTAs also mean that everyone is on the same page as to how the shared reagents should...organizations to make policy decisions on research use agreements. The UBMTA, which is the MTA most plasmids at...department is responsible for managing research use agreements like MTAs.  Addgene does a lot to ensure that...tracking when biological reagents are shared — both at what time and with whom. Tracking these connections...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...classes, workshops, book clubs, seminars, and social events. Building a community science lab In the Fall...it. We started out by holding a few journal club events and soon realized that it was easy to find people...entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using nanopore ...sequencing at BosLab to perform proof-of-concept experiments for discovering novel therapeutics. Dave is a...optimizes plant cell culture and transgenesis he wants to share his knowledge with the community by teaching... will engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly...ecology but she became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast ...

  20. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...in silico. These algorithms are trained on vast amounts of data to calculate the relative immunogenicity... replicate the complexity of antigen processing events that take place within an antigen-presenting cell...conditions, etc. that can vary widely between recipients.   In vitro prediction of drug immunogenicity... to validate the findings from the in silico assessments, as well as analyze the contributing factors ...in-vitro models can be built that contain various components of the immune system. Figure 2: In-vitro...Potential Use of HLA Haplotypes to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org...Algorithm Infrastructure and a Vision for Future Enhancements. Frontiers in drug discovery, 2, 952326. https...
Showing: 401 - 420 of 1020 results