We narrowed to 766 results for: Ncl

...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...

... maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity..., they have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource...

...used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc 2, BioImageXD...to the properties of FPs themselves. These can include the potential to oligomerize, protein charge, and...

...gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction...using the Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: ...

...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...

...improvements? You could publish a paper that happens to include your improvements, or maybe even a methods paper...variety of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid...

...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...regions” filter unchecked so that these regions are included in the search to maximize the alignment length...

...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...

... have been developed based off of this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, ...innovative labeling strategies, several groups—including mine—have been revisiting the old chemistry of...

...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...

...and its RNA directed binding site. These methods include the fusion of an activation domain such as VP16... or tracrRNA sequences can also be modified to include RNA domains that recruit additional transcription...

...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...

...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...will simply express from whatever promoters were included in the vector (or are nearby in the host genome...

...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...in the detection of microbes. Some applications include measuring viable probiotics and detecting circulating...

...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....

... CRISPR complex much easier in various cells, including primary mouse cells. We believe that this system...sight in that Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop...

...antibiotic resistance develop? Microbes (bacteria included) are very skilled at the delicate art of survival... contributing to the development of resistance include (Figure 1): Over-prescription of antibiotics (...