We narrowed to 663 results for: PPL;
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TypeBlog Post...connection you made at the job fair when you formally apply for the position. This post on Business Insider ...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible...
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March for Science
TypeBlog Post...earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush Holt said...are split along party lines (18). Feeling like an apple will fall up instead of down when dropped will not... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ... -
Five Popular Model Organisms, Part 2
TypeBlog Post...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently labelled ssDNA substrates. Upon ...fluorescence can be visualised. Another approach was to apply the same concept to lateral flow strips that contain... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower...lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán et al. 2022... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, ...is an umbrella term for a number of different applications that involve different types of small RNA molecules... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...choosing a lab, but almost all of these guidelines can apply to any workplace or job. It amazes me when the most... Is the work (topic and/or technical aspects) applicable to industry if this path interests you (e.g.... -
CRISPR 101: Off-Target Effects
TypeBlog Post...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...regions flanking the target site). The best applications for dsDNA inserts include: large insertions ...Uses in Recombineering Substrate Best Suited Application Recombination Frequency Special Considerations... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...sequencing is laborious, time consuming and cannot be applied to heterogenous populations (Bell et al. 2014)....Genotyping Options Technique Detection Limit Application Advantages Disadvantages GUIDE-Seq 0.1% Cell-based... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct comparative... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost... unedited strand (Chen et al., 2021). The team applied this approach to both the PE2 and PE3 systems, ... -
Technical Design of a Western Blot
TypeBlog Post...published antibodies, check the manuscript and supplementary figures for validation data, or follow the references...it includes validation data relevant to your application — and double-check that lot number while you’... -
New Western Blot Protocol Video!
TypeBlog Post... Western blot and share tips for this tricky application. You can check out all of our protocols ... -
It’s CRISPR Week at Addgene!
TypeBlog Post...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...