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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 985 results for: Por

Showing: 401 - 420 of 985 results

  1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...back in with the Addgene blog for future award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima ...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...during the pandemic and in the future, depends on support from the scientific community. Deposits of new ... be deposited into repositories, and financial support to maintain repositories’ capacity for storing,...all ways in which the scientific community can support repositories. Repositories are essential for the...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...will include, but is not limited to temporary housing, transportation, living expenses, and necessary reagents...visits an institution to perform research there temporarily. The length of stay could be from a month to ...forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it sounds right...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI...attention for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, ...., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887...

  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing... and the targeting guide RNA into cells by electroporation. Results from our lab and others (Lin et al...Kim et al 2014) have shown that this technique supports extremely high frequencies of genome cutting and...nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...

  7. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....

  8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...binding ability of its nanobody partner, so it’s important to insert AsLOV2 at a spot in the nanobody that...light to control signaling provides greater spatiotemporal resolution than pharmacological or genetic ...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary...

  9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...nuclease for your experiments, there are three important factors to consider: site accessibility, specificity...potential for off-target cleavage should be an important consideration during any experiment. Sensitivity...stringent PAM specificity. This can be especially important for base editing techniques, where Cas9 must be...many options! Comic by Maya Kostman. Another important consideration when choosing a Cas9 is balancing...

  11. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration...

  13. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...severely cold conditions. To understand their unique importance, let’s begin with a few basics.  Mesophiles vs...understanding and appreciating their biology. The importance of psychrophilic enzymes From an industrial perspective...decided to create a hybrid system where we could incorporate cold-tolerance inducing genes within a regular...scientist from Addgene. The talk highlighted the importance of reagent repositories and the myriad facilities...

  14. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...scientific conferences. Conferences provide the opportunity to meet people who are interested in the same...the conference. Large conferences have many opportunities but can be overwhelming as far as meeting new...the conference The conference may start in the airport. If you see someone traveling with a poster roll...friendly so stick with those. Don’t miss social opportunities, but don’t overdo it (some of my best studies...

  15. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...plants are the foundation of life on earth, and opportunities abound to build better fuels, feeds, foods, ...areas in plant biotechnology that are ripe with opportunity. We’ve decided to jump into one of those unexplored...out their mission of creating new forums and opportunities for open innovation in plant synthetic biology...build the plant synthetic biology community and to support more efficient engineering of a better tomorrow...

  16. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    .... But how do you find these elusive teachers, supporters and advisors? I tried to start a more formal ...for their development plans. They all found the support of the group to be intrinsic to their success. ... continue to meet regularly, providing ongoing support, advice and accountability for development goals...mentoring relationship.  Celebrate your successes and support each other as you transition to the next stage ...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...people can’t find you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For... you don’t add a personal note, you waste an opportunity to start things out right. Also, many people ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...had a chance to proof read. This might not be important for a good friend, but if you are making a professional...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ... because in vitro conditions did not optimally support growth of diverse species. These results further...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition...

  19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...2017). However, Acr inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument...versatility opens up the potential for spatial and temporal control in many genetic targeting and editing ...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...response to light, offers a way to spatially and temporally control cellular processes in vivo. Many photoactive...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...containing Cas9 and two different gRNAs, and electroporated them with an ssODN repair template in induced...in cases of disease prevention. However, it is important to consider that already-tested CRISPR alternatives...clinical applications could be considered. It is important to consider the question of broader applicability... large step forward in the CRISPR field, it’s important to remember that the study hasn’t achieved any...
Showing: 401 - 420 of 985 results