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...mesophilic systems are the psychrophilic enzymes.  The reason behind this isn’t really hard to guess. Think about...for all the Indian iGEM teams to interact with personnel from the industry and brainstorm with each other...

...inactive state, and cheap. Unless there’s a specific reason you can’t use it for your ELISA and Western, I’... Rachel Leeson...

...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu ...

...never picking up the phone you don’t get to know a person. These interactions could either help you in your...an MBA in finance and a B.S. in accounting from Babson College. He is a certified public accountant (CPA...

...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...

...started working in a new lab and I was the only person in the lab. When I saw a request for an Addgene...scientific conference, whether you are attending in person or monitoring that conference via social media....

...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...

...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield...

...talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you take time... Rachel Leeson...

...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’...

...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

...interest and its localization in the membrane. The lesson we learned from these studies is that the tag protein...Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of ...

...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...

...entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative ...  Rachel Leeson...

...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies...

...the first author of this article, Dr. Douglas Richardson, recently contributed a relevant post to the ...Addgene blog. Find the SRpHi Biosensors here. Richardson et al. Nat Communications. 2017. PubMed PMID:...

...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working...

...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the...