We narrowed to 728 results for: STO;
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TypeBlog Post...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want...
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Hot Plasmids - October 2020
TypeBlog Post...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for observation in live cells.... -
CRISPR in the Clinic
TypeBlog Post...Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause at the... Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored... -
Hot Plasmids: Summer 2025
TypeBlog Post... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...purification, and titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service... -
Degrading DNA with Cascade-Cas3
TypeBlog Post... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...HDR. If the max CFD score is less than 0.03, it stops mutating. (This happens often because the HDR inserted...will check the CFD score. If it is below 0.03, it stops mutating. (Note: the most common synonym in the ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...late 1960s during research at hot springs in Yellowstone National Park. In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that...Plasmids for Mammalian Systems Learn about CRISPR History ... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...see our plasmid collection from Sue Jaspersen (Stowers Institute) and Mark Prescott (Monash University...ImageJ plugins JACoP and Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...