We narrowed to 452 results for: SUP

1. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

2. Twenty Years of Sharing: Expanding Accessibility

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   Blog Post

   ... resources As Addgene built out our scientific support services, we realized that another availability...

3. Hot Plasmids Spring 2024

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   Blog Post

   ...associated with viral vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing...

4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

6. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. Daily medium...

7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ..., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments ...

8. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...cables. One huge advantage of using rats is their superiority as behavioral models. Beyond rodents, optogenetics...

9. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ...193–208 . https://doi.org/10.3390/antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul...

10. Five Popular Model Organisms, Part 2

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    Blog Post

    ...genome-scale functional genomics study in bacteria with superior performance. Nature Communications, 9(1). https...

11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post

    ...indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently labelled ssDNA substrates. Upon ...

12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

    ...proxy for dimerization (Shaner et al., 2008 - supplementary figure C). However, these assays are qualitative...

13. Plasmids 101: Biotinylation

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    Blog Post

    ...plague these animals even if their diet was being supplemented with biotin. Thus something in the egg white...

14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus. Sci Rep. 2015 Jun 2;5:10833...

15. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research in human embryos. Nature. 2015 Dec...

16. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...re looking at institutes that have the means to support your work. For example, if your work requires specialized...

17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman...

18. Guide to Using Pooled Libraries

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    Guide

    ... guessed from their name, pooled libraries are supplied as a mixed population of plasmids in a single ...

19. Centrifugation

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    Protocol

    ...sample while the liquid portion is known as the supernatant. Depending on your experiment, you may be required...required to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many...

20. A Control for All Seasons

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    Blog Post

    ...binding  Primary antibody specificity is further supported by a pre-immune serum or isotype-specific control...