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We narrowed to 887 results for: TRI

Showing: 401 - 420 of 887 results

  4. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...explanation of the assay is depicted in figure 1. The strict correlation between the fluorescent protein of ...

  5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...works. But, those darn cancers have revealed many tricks up their sleeves and most patients still don’t ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...

  6. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

  7. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ... a biotech startup company? Would you like to contribute advice on how to effectively raise money and/... Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and...the Biotech Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments...

  8. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...real-time inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists ...electrophysiology, a technique that’s used to measure electric current of ion channels in living cells. Multiple...-stimulate” their VTA dopaminergic neurons by triggering a burst of light that could activate ChRger2 ...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector ...work done previously, borrow small aliquots of restriction enzymes for one-off projects, or even save yourself...

  10. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "...interleukin-6 (Witkiewicz et al., 2009) which contribute to the development and spread of cancer cells... scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

  12. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... held from 22-24 October. This blog has been contributed by guest blogger and Future of Research Symposium...the academic labour market. Her many notable contributions in this area include publishing the book ‘How...How Economics Shapes Science’ and contributing to the NIH’s Biomedical Research Workforce Working Group...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends ...organs, which then return information such as nutritional status. Within the animal kingdom, this information...300 different coactivator proteins individually contribute to cell regulation in a coherent manner. Because...

  14. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing if you can...Cloning specifics Fragmid uses Bbsl, a Type II restriction enzyme that cleaves outside of its recognition..., 93% had the correct banding pattern in the restriction enzyme digest. They sent 82 of the correctly ...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    ...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...Education Project (pgEd), where I currently work, strives to broaden the conversations about genetics and...implications of genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic...

  16. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...used to reveal brain circuitry, and continue to contribute answers to the complex questions of how the brain...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell types...types, pairs of synthetic promoter can be used to restrict expression to a single cell type based on the ...cell-type specific promoters could be an easier way to restrict expression of a gene interest in an animal model...

  18. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...Scholar library. You can add hashtags to library entries, which can help you organize and search for papers...number of times a paper is shared online, or its altmetric score, is also displayed in your feed. By clicking...us know if you if you have any favorite tips and tricks for navigating the always growing sea of scientific...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named...with a FLAG tag (!), but did you know that we distribute ready-to-use recombinant antibodies as well? ...anti-FLAG antibody, both of which are also available in trial sizes. Addgene recommends these recombinant antibodies...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute...regions of high homology to the gene with the cut, we trick the cell into using the introduced donor plasmid...Allen Institute for Cell Science. This post was contributed by the gene editing team at the Allen Institute...
Showing: 401 - 420 of 887 results