We narrowed to 752 results for: UTY

...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...

...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of...

... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

...this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ...

...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...

..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...

...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https...

... research focuses on protein innovations for therapeutic and vaccine design. We all want tools that expand...of Click Biology is made possible through the utilization and development of genetically engineered components...

...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-...

...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ...

...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific...

... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...worries about off target effects in applied and/or therapeutic settings. Will combining the different mutations...

... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

...literature searching and reading a regular part of your routine. Set a goal to read a certain number of papers ...though you may need to be on a network with an institutional subscription), archived on PubMed Central, or...