We narrowed to 749 results for: UTY
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TypeBlog Post... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...
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GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...more effectively used in a technology or as a therapeutic. A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ... -
Viral Vectors 101: Types of viruses
TypeBlog Post...gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender.... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-... -
dTAG - You're it!
TypeBlog Post..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules... -
Addgene: The First Twenty Years
TypeBlog Post...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ... -
Antibodies 101: Fab Fragments
TypeBlog Post...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https... -
To Each HIS Own
TypeBlog Post...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ... -
All in a Twist: dsRNA
TypeBlog Post...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...worries about off target effects in applied and/or therapeutic settings. Will combining the different mutations... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages Resources at Addgene... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...literature searching and reading a regular part of your routine. Set a goal to read a certain number of papers ...though you may need to be on a network with an institutional subscription), archived on PubMed Central, or... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...through programming cells. In the context of therapeutics, if you want to program a cell to identify and... far nothing that has worked. I don’t have the solution. Our paper shows we can easily make orthogonal...