We narrowed to 743 results for: UTY;

1. Behind the scenes: Addgene’s new search engine and more

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   ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

2. Building and Validating Recombinant Antibodies: A Case Study

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   ... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...

3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ...

5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

6. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   ...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...

7. Addgene: The First Twenty Years

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   ...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...

8. Antibodies 101: Fab Fragments

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   ...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https...

9. To Each HIS Own

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   ...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ...

10. All in a Twist: dsRNA

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    ...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

11. RaPID Detection of RNA-protein Interactions

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    ...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-...

12. dTAG - You're it!

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    ..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific...

14. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...worries about off target effects in applied and/or therapeutic settings. Will combining the different mutations...

15. New Tool for Lineage Tracing: The ClonTracer Library

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    ... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...literature searching and reading a regular part of your routine. Set a goal to read a certain number of papers ...though you may need to be on a network with an institutional subscription), archived on PubMed Central, or...

17. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...through programming cells. In the context of therapeutics, if you want to program a cell to identify and... far nothing that has worked. I don’t have the solution. Our paper shows we can easily make orthogonal...

18. Plasmids 101: The Wide World of Natural Plasmids

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    ...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...or licenses that could prohibit Addgene from distributing the plasmid, select “yes” from the drop-down...but can accept sequence files in any format. Distribution status for your plasmids: Hold for Publication...

20. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are... in freshwater ponds, provide a good potential solution to this problem. Planarians are easy to buy, cultivate...