We narrowed to 770 results for: VARS
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TypeBlog Post...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...
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New Educational Resource: The Addgene Videos Page
TypeBlog Post... feature step-by-step instructions to navigate various lab processes. Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene. You’ll hear all about their... -
Call for Guest Bloggers
TypeBlog Post...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog Post...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog Post...spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...Seeding Labs Plasmid grant and is working on a variety of projects in Ghana. Special thanks to our... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...’s molecular biology work has focused on using various cloning methods and tools to construct chimeric...like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial... -
Management for Scientists: Seeking Feedback
TypeBlog Post...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ... -
What's New in CRISPR - March 2020
TypeBlog Post...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact ... this feature will draw attention to the great variety of plasmids in the repository. And last, but certainly... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase... -
Plasmids 101: What is a plasmid?
TypeBlog Post...into a vector, scientists may utilize one of a variety of cloning methods (restriction enzyme, ligation...research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid use... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence... -
SunTag and Fluorescent Imaging
TypeBlog Post... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...in moderate thermophiles. These evolved SpCas9 variants have improved activity on NAG PAMS and reduced... Various Addgenies... -
New Viral Vectors - Fall 2024
TypeBlog Post...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)? AAV Variables that Matter... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...