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We narrowed to 409 results for: expression vector

Showing: 401 - 409 of 409 results

  1. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...

  2. Chemogenetics Guide

    Type
    Guide
    ... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...which they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic...chemogenetic receptors. FLEx vectors block expression in the absence of Cre and are especially useful in AAV...Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors blog! Download our chemogenetics.... The opsin may excite or inhibit the neurons expressing the LMO, depending on its biophysical properties...more than one domain, both of which need to be expressed. However, neuronal control through PSAMs and LMOs...

  3. Optogenetics Guide

    Type
    Guide
    ...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...fiber. Using viral vectors for opsin delivery results in more localized expression of the opsin. Depending...site of the viral vector, rather than in all cells. For example, using a viral vector with a floxed opsin...procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of...as those described below, can be targeted and expressed in specific subsets of neurons, allowing precise...population is manipulated in a given experiment: the expression of the opsin and the area that is being illuminated...for example the VGAT-ChR2 mouse, where ChR2 is expressed under the control of the vesicular gamma aminobutyric...

  4. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  5. Promoters

    Type
    Guide
    ...initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase...where in the organism your gene of interest is expressed. Summary Promoters are about 100-1000 base pairs...Eukaryotic Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian...Constitutive Human U6 nuclear promoter for small RNA expression UAS Specific Drosophila promoter containing Gal4...Bacterial Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA...

  6. Cloning

    Type
    Guide
    ...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase enzymes...a simple and efficient cloning method, gateway vectors and recombination enzymes can be quite expensive...comes from a pre-cut, linear, cloning ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ ...ready, and it is not feasible to create a TOPO® vector yourself. Additionally, the efficiency can vary...overhangs of 10-12 base pairs on the 5' end of both the vector and insert. These overhangs can easily anneal creating...

  7. Science Guides

    Type
    Guide
    ...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...given library have the same backbone, but they express or target different genes. Some libraries cover...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ...given library have the same backbone, but they express or target different genes. Some libraries cover...like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...
Showing: 401 - 409 of 409 results