We narrowed to 405 results for: expression vector

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

...entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors deposited...them into plasmids for replication and protein expression. There are many methods that allow you to move...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...shuttled into any compatible Gateway destination vector, which contains attR sites, via LR clonase enzymes...a simple and efficient cloning method, Gateway vectors and recombination enzymes can be quite expensive...donor , Gateway entry , and Gateway destination vectors with different promoters, tags, selection markers...comes from a pre-cut, linear, cloning-ready TOPO vector that has a DNA topoisomerase I covalently bound...

...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...given library have the same backbone, but they express or target different genes. Some libraries cover...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...

...given library have the same backbone, but they express or target different genes. Some libraries cover...like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...

...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...