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We narrowed to 1,050 results for: ins;

Showing: 401 - 420 of 1050 results

  1. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...pursue leadership opportunities at the local, institutional, regional, and national levels. These skills... But how does one gain such experience? Your institution may offer occasional workshops on leadership ...Harvard Medical School and then Boston University (institutions where I’ve worked as a postdoc). I soon realized...that many of the issues faced by postdocs and institutions are not local but are national issues. I applied...requirements of the new Board Members. In some instances I felt like I was going back to previous formats...every year it organized a “Committee Leadership Institute” (CLI) where each Committee Chair and Vice Chairs...the Burroughs Wellcome Trust. These “leadership institute’ weekends would entail intense training to develop...

  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...detector plants, we expected to see that same anger. Instead, social media comments were more often along the...that.” That was interesting. If people could be against GMOs as food but support GMOs that result in a ...business training. The money is a drop in the bucket against the funds needed to build a flower that changes...business enterprises, he can be found climbing mountains or playing with his two cats. Keira Havens grew...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    ...something done not by people wearing a lab coat, but instead by people wearing business suits discussing multimillion...that means you don’t. In this mindset, one of us wins, the other loses. Although this approach to negotiation...winner and a loser when negotiating, what if we instead approached it as a creative problem-solving exercise...point you toward a creative solution where everyone wins. What are the underlying reasons for this person...some data for an upcoming conference deadline. Instead of arguing over who gets the spot (a zero sum result...Coming up with creative solutions where everyone wins can initially feel more difficult because of the...can I offer them to make it a win-win situation instead of just extra work for them? As a business development...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...stem from the heart beating at an irregular or insufficient level. The heartbeat – a muscle contraction ...structurally into 2 main groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors...that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport, not ...membrane due to the addition of several targeting domains. The quencher and voltage detector in this system...viruses also have various serotypes – the surface proteins that confer affinity for a target – and this serotype...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them for targeting... Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ...

  5. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... sequences, such as mismatches, deletions, or insertions. We use blastp or blastx to compare our sequencing...database “Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...Non-redundant protein sequences (nr)” database as it contains the largest number of protein sequences. Just ...customization. Similar to nucleotide sequences, proteins often have repeated or highly homologous regions... and free up more time for using your plasmids instead! All BLAST images are modified screen shots from...101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality Control Learn about...

  6. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins. The most common method for checking...to be free of contaminants, such as bacterial endotoxins, that could affect your culture’s viability. ...plasmids – If you’re extracting plasmids from yeast, instead of bacteria, a few manufacturers (such as Zymo ...struggling with a purification, reading the full instruction manual will help explain the rationale behind... helpful to select a kit with easy-to-follow instructions and extensive references for troubleshooting... compare kits at a quick glance, Table 3 below contains details regarding kits from a variety of manufacturers...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

  7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...NanoDrop. The principles will apply to similar instruments from other companies, like the Implen NP80 or...Figure 1A). Thanks to the Beer–Lambert law, the instrument can calculate a sample’s nucleic acid concentration...downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own... trying to remove from your sample is protein. Proteins absorb light to varying degrees across the UV ...Cantor, 2019). The 280 nm peak is more specific to proteins and why researchers traditionally use the A260...will yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your...! Be sure to review resources on the specific instrument you use and check out some of the references ...

  8. Deck the Lab 2022!

    Type
    Blog Post
    ... contestants!           View this post on Instagram                     A post shared by Xi Chen...December 12, 2022          View this post on Instagram                     A post shared by 草方 (@gracie_fang...take photos, and then share them on Twitter or Instagram with #DeckTheLab and tagging us, @addgene to be...get you out of.           View this post on Instagram                     A post shared by Xi Chen...picture or video for either contest on Twitter or Instagram by December 31, 2022.  Follow @Addgene, use the...and reveal the winning entries on Twitter and Instagram! Happy decorating, all, and to all a good light...

  9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...reprogramming efficiency through the expression of a shRNA against p53, a modified reprogramming factor cocktail, ...are preferred. This is to avoid the chances of insertional mutagenesis, which is the integration of transgenes...disruption of functional genes at the site of insertion and which can potentially promote tumorigenesis... been recorded in past gene therapy trials and remains a safety concern in the clinical field [4]. Three...Antigen-1 (oriP-EBNA-1) system where each vector contains a viral origin of DNA replication, OriP and the... pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and shRNA against p53 pCXWB-EBNA1 37624 transient EBNA-1   ...Road Ahead." Journal of Alzheimer's disease & Parkinsonism 6.5 (2016). PubMed PMID: 27882265. PubMed Central...

  10. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...get online and take a look at the company or institution’s career page, where you’re likely to find current...conference or job fair, or it may be someone at your institution’s career center. Alternatively, it might even ...other graduate students and post-docs from your institution have gone, and try sending them a professional... professional development, not count on your institution, program, or adviser to do it for you. Expose...if one exists. Get online! Review company and institution career pages. Make yourself visible on LinkedIn... Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...diagnosed this year). Research efforts in mesothelioma instead rely upon private donations. Many people think ...However, there is no question that asbestos exposure remains the single-most important factor in developing ...do not work very well. The best option in such instances is palliative care, which helps patients manage...detection techniques and therapies emerge into mainstream use, they will have a much greater impact on ... good detection and treatment get, there still remains the problem of prevention. Asbestos exists pretty... and the president of the organization, Linda Reinstein – whose husband passed away from mesothelioma ...with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016 in the U.S. Senate...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistant to antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally...resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species’ resistance...possess cell walls. As a result, Mycoplasma are intrinsically resistant to this class of antibiotic. Acquired...DNA left behind after another bacterium died for instance). These three mechanisms of acquired drug resistance...be unharmed by the compound it produces (think intrinsic resistance), but surrounding microbes would be... time is needed for the drug to work… if the instructions for treatment aren’t followed closely, you could...in the Lab Probe Phage Genomes for Host Binding Proteins Learn How to Use Our Microbiology Pages Additional...

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Singletons in their bins.  The next component of Binning Singletons is when we bring all the bins together at ...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...their own labs.  Fried chicken with a side of inspiration  I look back at the meeting with such fondness...for DNA that group similar sequences together in bins, we thought that binning Singletons together would... and time mentors spend with Singletons in their bins. We also sought expertise from many with experience...media. Joe is a lapsed marathon runner, father of twins (AND a singleton), and is proudly "too weird to ...

  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...frequency of events, instead of relying on clustered dots. In a contour plot, each line contains the same number...machine reads/interprets the signals, the axis is instead divided into positive and negative signal at a ... a ‘gate’; it tells the machine that everything inside the gate is one population and everything outside...which measure light that “scatters” past the cell, instead of fluorescent markers. There are various types...to fluorescent markers and/or cellular dyes and stains. These will be used in a series of one- or two-...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels (which had therefore been ... light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential labeling by TurboID...2023 bioRxiv (B-C). They were able to label proteins moving to and from the nucleus, ER, mitochondria...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...approach, which focuses on validating antibodies against markers of the human blood leukocyte populations...cases, we would recommend a knockdown approach instead. In this approach, you’re looking for an antibody...permanent knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult...induce/suppress the expression of many related proteins, not just your protein of interest, which can ...interest, which can be difficult to find for many proteins. In practice, it was necessary for us to combine...primary blood leukocytes. The file shared also contains information about the labeling pattern of several...on human blood leukocytes, including antibodies against these markers. The 371 CD markers characterized...

  17. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral...FRET reporters are comprised of two fluorescent proteins with very specific spectral properties that limit... and the orientation of the donor and acceptor proteins, which can be difficult to predict. Slower Dephosphorylation...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...are only expressed when the targeting vector is inserted downstream of a functional promoter (gene traps...2008). While the biology behind this AAV effect remains unclear, it is likely not a coincidence that the...gene. pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene,... CRISPR-based approaches for the generation of knockins via homologous recombination with a homologous...application - the creation of sequence-specific knockins - for which the AAV approach is most well suited... CRISPR-based approaches for the generation of knockins, it is not always ideal. Because of these limitations...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...the ability to generate full libraries of mutant proteins. Researchers from the Whitehead lab developed ... Gibson Assembly: An easy-to-use technique that joins homologous DNA fragments in a single-tube reaction...using Gibson assembly is that the longer the oligo insert used, the more likely it will contain random synthesis... enzymes leads to a buildup of uracil and the insertion of uracil into newly synthesized DNA. This labeled...synthesized DNA strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a ...that the BbvCI enzymes nick the DNA strand that contains their respective recognition site. This means ... matter? Most of the time no, but there are 2 instances where it does. 1) When the backbone vector has...

  20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plasmid, into which the GOI is inserted, and a Ti "helper" plasmid, which contains Vir genes necessary for T-DNA...gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning required....Dicot Genome Editing Kit from the Stuttmann lab contains a variety of Agrobacterium-compatible, Cas9 containing... T-DNA processing and insertion into the plant genome. B) The Ti plasmid and helper are transformed into...the Vir genes on the helper plasmid and used to insert the intervening region into the plant genome. Created...- promoters used, precise protein sequences or domains, and methods of delivery - the underlying technique...
Showing: 401 - 420 of 1050 results