...about using non-homologous end-joining (NHEJ) to make deletions or homology-directed repair (HDR) to create...very small homology regions (5-25 bp) for repair, making it easier to construct targeting vectors. Addgene...downregulated, MMEJ represents another method for making targeted modifications. A recent publication by...

... does making transgenic organisms with Cas9 risk creating a gene drive? The simplest way to make a gene...Institute and Harvard Medical School. Scientists making transgenic organisms with Cas9 should be aware ...

...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...Maity et al., 2015). If your antibody is not IgG, make sure to look up the setting for your isotype.  Antibody...

...researchers need to reflect upon before they attempt to make essentialist connections between biology and ancestral...Map (DepMap), a cancer dependency database used to make this information accessible to the research community... at all. This unexpected discovery, which didn’t make sense in any kind of biological framework, suggested...

...below). Addgene scientists inspect each sample and make sure they have been barcoded with a unique ID number...therefore particular sample is missing so that we can make a new tube from a replicate sample. The transformation...replicate and retain the plasmid. This allows us to make unlimited copies of each plasmid so that we never...

...You will often hear the term ‘grant writer’, but make no mistake, researching, winning, and effectively...writing. In fact, many grant applications and grant makers have moved away from long written proposal narratives...relationships with foundations and government grantmakers. We keep up-to-date on the changing guidelines...

... bench. Specifically, roles in technical support make excellent use of the skills developed by life science...advantageous to speak multiple languages - that makes communication with customers from around the world...convenient alternative for asking a quick question or making initial contact, but is less suitable for troubleshooting...

...well-researched field, you may need to get specific to make sure your article doesn’t turn into a textbook. ... short bursts (even twenty minutes was enough to make some progress). For most researchers, it’s not always... into something cohesive-- change the sentences, make it flow, and start telling the story. Like any editing...

...zebrafish embryo develops outside of the mother, making them ideal for studying vertebrate embryogenesis...zebrafish To highlight some of the features that make zebrafish useful, I’ll outline two recent lineage...unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow” fish lines have been...

...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene...come up with a list of things to consider as you make this transition. Of course, a “desk job” could entail...

...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...also helped us find areas for improvement. We will make a greater effort in the future to match people within...Binning Singletons is its own organization, working to make scientific meetings more productive for attendees...

...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import... to increase accessibility to our repository by making it easier to find materials and learn about them... know we will continue to ask “What can we do to make our materials both available and accessible to scientists...

...truncated forms that limit its functionality. To make biophysical and structural studies more feasible...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

...false positives observed in the cloning process, making it easier to produce the constructs you need. Expression...affecting its translation initiation and instead makes initiation heavily dependent upon the core of a ...to simplify the protein purification process by making it easy for a researcher to quickly generate N-...

...specific target, and the molecular processes that make aptamer biology a powerful tool. In this post, we...dependency on magnesium and higher thermal stability, making it a potentially more useful tool for in vivo studies...interaction between Corn and its small molecule makes Corn more photostable than Spinach and Broccoli,...

...Being smaller means scFvs are easier and cheaper to make because they can be expressed in bacteria, while...with other protein domains, much like lego pieces, makes them better suited than antibodies to be used as...T cell receptors and are expressed on T cells to make CAR T cells. The extracellular scFv portion of the...

...sometimes it can be a big challenge. But ask any filmmaker or videographer and they’ll tell you, a lot of...Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing Board Control Center...: There were still a few adjustments I wanted to make to ensure smooth filming in the future, but we were...

...target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In...sequence was inserted or deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you...issue, you may need to select an alternative gRNA. Make sure to check out our validated gRNAs to avoid rolling...

...commitment. Another advantage is that this process makes it easy to generate wild type and mutant constructs...content, contains a lot of repeats--any of which could make the PCR step difficult--OR there is no convenient...rather than using a restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a ...

...Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers...normal function. Even low-frequency editing could make a huge difference in DMD, and it’s estimated that...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome...