We narrowed to 536 results for: val

1. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...project. README: The README.txt document is invaluable. In the top directory of every experiment, immediately...

2. Human Germline Editing Using CRISPR

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   Blog Post

   ...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

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   Blog Post

   ...phosphorylation status of MEK1/2 was tracked at 5 minute intervals via Western blot. Phosphorylation of MEK1/2 peaks...

4. Working with Nuclear Receptors

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   Blog Post

   ...contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance...

5. 5 Steps to Writing Grant Proposals for Grad Students

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   Blog Post

   ...challenging because it’s hard to determine the exact value of costs. It is important to be aware of supply ...

6. Career Coaching for Scientists: Why and Where Do I Find One?

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   Blog Post

   ...under-represented minorities. Her online content is of great value. I have never been in contact with Aida Baida Gil...

7. Plasmids 101: Cre-lox

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   Blog Post

   ...will be disrupted or deleted. Selection marker removal – in conventional mouse targeting, targeted clones...

8. CRISPRainbow and Genome Visualization

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   Blog Post

   ...targets ~2 Mbp apart, they noticed that they could evaluate the degree of chromatin compaction even for this...

9. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...three years as a post-doc in the Target ID and Validation Group and says that she decided to do a post-...

10. Plasmid Tools for Microbiome Studies

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    Blog Post

    ... clinically relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison...

11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR...

12. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...irreproducibility. Use your network well to seek out valuable and impactful opportunities - There are many brilliant...

13. Celebrating 15 Years of Scientific Sharing

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    Blog Post

    ...depositors. We regularly hear how much they see the value in depositing and they are increasingly seeking ...

14. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch...

15. The time and cost required to make a plasmid

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    Blog Post

    ...today. Finally, this doesn’t account for the most valuable component in science – the time spent by the scientists...

16. To Each HIS Own

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    Blog Post

    ...to expose the tag and then refolded by gradual removal of the denaturant. Not all proteins will refold...

17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell tracing using the transgenic ...

18. All in a Twist: dsRNA

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    Blog Post

    ...or low levels, possibly due to dysregulation of removal mechanisms, an array of diseases can result (Fanconi...

19. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ... itself is being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA sequences...

20. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...evolution, because channelrhodopsins are typically evaluated with low-throughput methods such as patch-clamp...