SunTag and Fluorescent Imaging
Type
Blog Post
...traditional FP fusions can also affect protein activity, half-life, or localization, these concerns are...
We narrowed to 580 results for: vit;
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The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post...“blue flame” status. We’re proud to honor the creativity, passion, and dedication to sharing epitomized... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer off-target effects... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...reporter genes for in vivo imaging Following in vitro characterization, Bourdeau et al performed a proof-of-concept... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...understood, it is believed that the exonuclease activity of the polymerase generates sticky ends in the... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post... produce compounds with various antimicrobial activities (1). She has developed a fast endophyte screening... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes At this point... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post... TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists could... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...element (RBE) in the ITRs, uses its endonuclease activity to generates a ssDNA substrate which is then nicked... -
Hot Plasmids - October 2022
TypeBlog Post...authors show that HcKCR1 and HcKCR2 have high selectivity for K+ over Na+ and rapid photocurrent kinetics... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...with a little mutagenic assistance). Quicker CAST activity allowed certain mutated phages to outcompete their... -
An Addgene Summer Internship
TypeBlog Post...presented my research to Addgenies and friends. These invited guests came to see the research from all the high... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...Isaacman-Beck et al., 2019. Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic... -
Plasmids 101: What is a plasmid?
TypeBlog Post...Region Drives transcription of the target gene. Vital component for expression vectors: determines which... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in... -
Advice for Choosing a Research Project
TypeBlog Post...discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What is your ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable... -
The Strength of Story Telling
TypeBlog Post...address far and wide, keep your website up to date, invite people to ask questions, be approachable. Engage... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved...